Grappel S F, Blank F, Bishop C T
J Bacteriol. 1968 Apr;95(4):1238-42. doi: 10.1128/jb.95.4.1238-1242.1968.
The contribution of terminal galactofuranose residues to the antigenic specificity and to cross-reactivity of galactomannans isolated from five species of dermatophytes, Microsporum quinckeanum, Trichophyton granulosum, T. interdigitale, T. rubrum, and T. schoenleinii, was investigated. Galactofuranose units were removed from galactomannans I and galactomannans II by mild acid hydrolysis. The resulting mannans were tested for serological reactivity with rabbit antiserum to M. quinckeanum by qualitative precipitation in gel and by quantitative complement-fixation analyses. Our results showed that, with this antiserum, the galactofuranose residues contributed greatly to the antigenic specificity and to cross-reactivity of the galactomannans II, but these residues were less significant as antigenic determinants in the galactomannans I. We have shown that mannans isolated from three Candida species reacted with rabbit antiserum to M. quinckeanum.
研究了末端呋喃半乳糖残基对从五种皮肤癣菌（昆克须癣毛癣菌、颗粒状毛癣菌、指间毛癣菌、红色毛癣菌和许兰毛癣菌）分离得到的半乳甘露聚糖的抗原特异性和交叉反应性的贡献。通过温和酸水解从半乳甘露聚糖I和半乳甘露聚糖II中去除呋喃半乳糖单元。通过凝胶定性沉淀和定量补体结合分析，测试所得甘露聚糖与兔抗昆克须癣毛癣菌抗血清的血清学反应性。我们的结果表明，对于这种抗血清，呋喃半乳糖残基对半乳甘露聚糖II的抗原特异性和交叉反应性有很大贡献，但这些残基在半乳甘露聚糖I中作为抗原决定簇的意义较小。我们已经表明，从三种念珠菌属分离得到的甘露聚糖与兔抗昆克须癣毛癣菌抗血清发生反应。
