Lynch K L, Moskowitz M
J Bacteriol. 1968 Dec;96(6):1925-30. doi: 10.1128/jb.96.6.1925-1930.1968.
The relative ability of the calcium chelates of calcium disodium ethylenediaminetetraacetate (EDTA) and calcium trisodium ethylenetriaminepentaacetate (DTPA) to protect mice against lethal doses of Clostridium perfringens alpha-toxin was investigated. Their protective ability was assayed by the increase in survival time of mice which had been given large doses of toxin, and by determining the median protective dose of chelate that would protect mice against toxin at a minimum lethal dose of two. In both assay procedures, intraperitoneal, intravenous, and intracutaneous injections of toxin were utilized, and with each toxin injection route the protective ability of the chelate was determined with the three routes of injection. DTPA was 10 to 20 times more effective than EDTA with both types of assay procedure and with all injection routes. DTPA may be superior to EDTA as a protective agent not only because it binds zinc to a greater extent, but also because of its greater retention in the body and its ability to gain entrance into cells. It appears that DTPA may be of value as a therapeutic agent in gas-gangrene.
研究了乙二胺四乙酸二钠钙（EDTA）和三亚乙基四胺五乙酸三钠钙（DTPA）的钙螯合物保护小鼠免受致死剂量的产气荚膜梭菌α毒素侵害的相对能力。通过给予大剂量毒素的小鼠存活时间的延长以及确定能在最小致死剂量为两倍时保护小鼠免受毒素侵害的螯合物的半数保护剂量来测定它们的保护能力。在两种测定方法中，均采用腹腔内、静脉内和皮内注射毒素，并且对于每种毒素注射途径，通过三种注射途径来确定螯合物的保护能力。在两种测定方法和所有注射途径中，DTPA的效果比EDTA高10至20倍。DTPA作为一种保护剂可能优于EDTA，这不仅是因为它能更大量地结合锌，还因为它在体内的保留时间更长以及能够进入细胞。看来DTPA作为气性坏疽的治疗剂可能具有价值。
