Sidorova E V
Biull Eksp Biol Med. 1979 Oct;88(10):437-9.
Mice were immunized with Vi-antigen. Spleen cells, removed at different time after immunization, were cultivated in Eagle medium, containing glycine-14C. The biosynthesis of antibodies to Vi-antigens, autoantibodies to mouse IgG and antigen-dependent non-specific immunoglobulins (NigG) were determined by means of specific immunosorbents. Immunization of mice with Vi-antigen resulted in a sharp increase in antigen-dependent NIgG formation. Thus, this protein biosynthesis takes place not only during immunization with thymus-dependent antigens, but also in response to the thymus-independent antigen. It is shown that the synthesized antigen-dependent NIgG were not autoantibodies to self mouse IgG.
用Vi抗原免疫小鼠。在免疫后不同时间取出脾细胞，在含有甘氨酸-14C的伊格尔培养基中培养。通过特异性免疫吸附剂测定针对Vi抗原的抗体、针对小鼠IgG的自身抗体以及抗原依赖性非特异性免疫球蛋白（NIgG）的生物合成。用Vi抗原免疫小鼠导致抗原依赖性NIgG形成急剧增加。因此，这种蛋白质生物合成不仅发生在用胸腺依赖性抗原免疫期间，也发生在对胸腺非依赖性抗原的反应中。结果表明，合成的抗原依赖性NIgG不是针对自身小鼠IgG的自身抗体。
