Eagon R G, Asbell M A
J Bacteriol. 1969 Feb;97(2):812-9. doi: 10.1128/jb.97.2.812-819.1969.
Evidence is presented that Zn(2+) and certain other multivalent cations inhibit the uptake and oxidation of substrates by Pseudomonas aeruginosa and by other bacterial cells. Tris(hydroxymethyl)aminomethane augmented this effect. Inhibition by these multivalent cations could be prevented by incubation of cellular suspensions of P. aeruginosa in phosphate buffer. In the case of other microorganisms, incubation in the presence of ethylenediaminetetraacetate also prevented the inhibition. Inhibition by Zn(2+) of the uptake and of the oxidation of substrates could be reversed by the addition of Mg(2+). Zn(2+) was shown to inhibit noncompetitively the uptake of the three substrates studied, l-alanine, d-glucose, and citrate.
有证据表明，锌离子（Zn²⁺）和某些其他多价阳离子会抑制铜绿假单胞菌及其他细菌细胞对底物的摄取和氧化。三（羟甲基）氨基甲烷增强了这种作用。通过将铜绿假单胞菌的细胞悬液在磷酸盐缓冲液中孵育，可以防止这些多价阳离子的抑制作用。对于其他微生物，在乙二胺四乙酸存在下孵育也可防止抑制作用。添加镁离子（Mg²⁺）可以逆转锌离子对底物摄取和氧化的抑制作用。已表明锌离子以非竞争性方式抑制所研究的三种底物（L-丙氨酸、D-葡萄糖和柠檬酸盐）的摄取。
