Hollis D G, Wiggins G L, Weaver R E
Appl Microbiol. 1969 Jan;17(1):71-7. doi: 10.1128/am.17.1.71-77.1969.
The biochemical and serological characteristics of lactose-utilizing strains of Neisseria were determined. These organisms were found in the nasopharynx of man and grew well on Thayer-Martin Selective Medium. They were compared with N. meningitidis to ascertain whether they were variants of this species. Differences between the lactose-using strains and the recognized species of Neisseria were considered significant enough to warrant designation of a new species, Neisseria lactamicus. This group has not been widely recognized as being separate from N. meningitidis; therefore, the normal incidence and clinical significance of these organisms has not been fully established. These organisms are oxidase-positive and positive for beta-D-galactosidase activity; they demonstrate fermentation in King Oxidation-Fermentation Medium; and they produce acid from only glucose, lactose, and maltose, of the 27 substrates incorporated in Cystine Trypticase Agar. Individual strains vary in their ability to grow on Nutrient Agar at both 25 and 37 C and in their pigmentation on Loeffler Medium. Results indicated that these organisms are serologically distinct from the N. meningitidis serogroups. Only 34 of 116 strains of N. lactamicus were smooth and could be tested by slide agglutination. None of the 34 could be grouped as N. meningitidis group A, B, C, D, X, Y, or Z. Thirty-one of these strains could, however, be specifically grouped with antisera prepared with N. lactamicus strains. Cross absorptions confirmed that N. lactamicus is serologically distinguishable from N. meningitidis.
对利用乳糖的奈瑟菌菌株的生化和血清学特征进行了测定。这些微生物存在于人类鼻咽部，在Thayer-Martin选择性培养基上生长良好。将它们与脑膜炎奈瑟菌进行比较，以确定它们是否为该物种的变种。利用乳糖的菌株与公认的奈瑟菌物种之间的差异被认为足够显著，足以指定一个新物种——产乳酸奈瑟菌。该菌群尚未被广泛认可为与脑膜炎奈瑟菌不同；因此，这些微生物的正常发病率和临床意义尚未完全明确。这些微生物氧化酶阳性，β-D-半乳糖苷酶活性阳性；它们在King氧化发酵培养基中表现出发酵；在胱氨酸胰蛋白酶琼脂中所含的27种底物中，它们仅从葡萄糖、乳糖和麦芽糖中产酸。各菌株在25℃和37℃下在营养琼脂上的生长能力以及在Loeffler培养基上的色素沉着各不相同。结果表明，这些微生物在血清学上与脑膜炎奈瑟菌血清群不同。116株产乳酸奈瑟菌中只有34株表面光滑，可以通过玻片凝集试验进行检测。这34株中没有一株可归为A、B、C、D、X、Y或Z群脑膜炎奈瑟菌。然而，其中31株可以用产乳酸奈瑟菌菌株制备的抗血清进行特异性分组。交叉吸收证实产乳酸奈瑟菌在血清学上可与脑膜炎奈瑟菌区分开来。
