Mackaness G B
J Exp Med. 1969 May 1;129(5):973-92. doi: 10.1084/jem.129.5.973.
It has been shown that the immune response of mice to infection with L. monocytogenes gives rise to a population of immunologically committed lymphoid cells which have the capacity to confer protection and a proportionate level of delayed-type hypersensitivity upon normal recipients. The cells were most numerous in the spleen on the 6th or 7th day of infection, but persisted for at least 20 days. Further study revealed that the immune cells must be alive in order to confer protection, and free to multiply in the tissues of the recipient if they are to provide maximum resistance to a challenge infection. The antibacterial resistance conferred with immune lymphoid cells is not due to antibacterial antibody; it is mediated indirectly through the macrophages of the recipient. These become activated by a process which appears to depend upon some form of specific interaction between the immune lymphoid cells and the infecting organism. This was deduced from the finding that immune lymphoid cells from BCG-immunized donors, which were highly but nonspecifically resistant to Listeria, failed to protect normal recipients against a Listeria challenge unless the recipients were also injected with an eliciting dose of BCG. The peritoneal macrophages of animals so treated developed the morphology and microbicidal features of activated macrophages. It is inferred that acquired resistance depends upon the activation of host macrophages through a product resulting from specific interaction between sensitized lymphoid cells and the organism or or its antigenic products. Discussion is also made of the possibility that activation of macrophages could be dependent upon antigenic stimulation of macrophages sensitized by a cytophilic antibody.
业已表明，小鼠对单核细胞增生李斯特菌感染的免疫反应会产生一群免疫致敏淋巴细胞，这些细胞有能力赋予正常受体保护作用以及相应水平的迟发型超敏反应。在感染的第6天或第7天，脾脏中的这类细胞数量最多，但至少持续存在20天。进一步研究表明，免疫细胞必须存活才能赋予保护作用，并且如果要对感染攻击提供最大抵抗力，它们必须能在受体组织中自由增殖。免疫淋巴细胞赋予的抗菌抵抗力并非源于抗菌抗体；它是通过受体的巨噬细胞间接介导的。这些巨噬细胞通过一个似乎依赖于免疫淋巴细胞与感染生物体之间某种形式的特异性相互作用的过程而被激活。这是从以下发现推断出来的：来自卡介苗免疫供体的免疫淋巴细胞，对李斯特菌具有高度但非特异性的抗性，除非受体也注射了诱导剂量的卡介苗，否则这些细胞无法保护正常受体免受李斯特菌的攻击。经如此处理的动物的腹腔巨噬细胞呈现出活化巨噬细胞的形态和杀菌特征。据推测，获得性抗性取决于通过致敏淋巴细胞与生物体或其抗原产物之间的特异性相互作用所产生的一种产物对宿主巨噬细胞的激活。还讨论了巨噬细胞的激活可能依赖于由嗜细胞抗体致敏的巨噬细胞的抗原刺激的可能性。
