Kammer H
Appl Microbiol. 1969 Apr;17(4):524-7. doi: 10.1128/am.17.4.524-527.1969.
An improved method for increasing cell yield from animal tissues is described. The current technique differs from the classical, primarily in that the tissues were perfused with trypsin in situ and cell dispersal was completed by trypsinization either at 4 or 37 C but without prior mincing of the tissue. Increased yields of 75% were obtained. Mechanical damage to tissue by mincing was presumed to be the greatest single source of injury to cells. The adoption of the trypsin perfusion technique reduced the number of animals needed to prepare an equivalent quantity of tissue culture.
本文描述了一种提高动物组织细胞产量的改进方法。当前技术与传统技术不同，主要在于组织在原位用胰蛋白酶灌注，细胞分散通过在4℃或37℃下进行胰蛋白酶消化完成，但无需事先切碎组织。产量提高了75%。切碎对组织造成的机械损伤被认为是对细胞最大的单一损伤来源。采用胰蛋白酶灌注技术减少了制备等量组织培养物所需的动物数量。
