用于动物组织胰蛋白酶消化的自动装置。
Automatic device for the trypsinization of animal tissues.
作者信息
Shipman C, Smith D F
出版信息
Appl Microbiol. 1972 Jan;23(1):188-9. doi: 10.1128/am.23.1.188-189.1972.
Abstract
Using a simple glass device, enzymatically released monodisperse cells can be separated and isolated from tissue fragments by means of a discontinuous fluid velocity gradient.
摘要
使用一种简单的玻璃装置，通过不连续的流体速度梯度，可以从组织碎片中分离并隔离酶促释放的单分散细胞。
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本文引用的文献
1
[Apparatus for continuous trypsination].[连续胰蛋白酶消化装置]
Ann Inst Pasteur (Paris). 1960 Jan;98:149-50.
2
Stabilized suspension of monkey kidney cells suitable for intercontinental shipment.适合洲际运输的稳定猴肾细胞悬液。
Proc Soc Exp Biol Med. 1955 Apr;88(4):676-8. doi: 10.3181/00379727-88-21692.
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TRYPSINIZATION OF ANIMAL TISSUES FOR CELL CULTURE: THEORETICAL CONSIDERATIONS AND AUTOMATIC APPARATUS.用于细胞培养的动物组织胰蛋白酶消化法：理论思考与自动装置
Appl Microbiol. 1964 Mar;12(2):115-21. doi: 10.1128/am.12.2.115-121.1964.
4
An apparatus for producing trypsinized monkey kidney cell suspensions.一种用于制备胰蛋白酶处理的猴肾细胞悬液的装置。
Virology. 1960 Feb;10:280-3. doi: 10.1016/0042-6822(60)90048-9.
5
Simplified method of dispersion of monkey kidney cells with trypsin.用胰蛋白酶分散猴肾细胞的简化方法。
Virology. 1956 Aug;2(4):575-6. doi: 10.1016/0042-6822(56)90013-7.
6
[Improved model of an apparatus for trysinization of tissues for cell cultures].[用于细胞培养的组织胰蛋白酶消化装置的改进模型]
Ann Inst Pasteur (Paris). 1956 Jul;91(1):103-6.
7
Trypsinization of monkey-kidney tissue: an automatic method for the preparation of cell suspensions.猴肾组织的胰蛋白酶消化：一种制备细胞悬液的自动化方法。
Bull World Health Organ. 1956;14(1):147-66.
8
Monolayer tissue cultures. I. Preparation and standardization of suspensions of trypsin-dispersed monkey kidney cells.单层组织培养。I. 胰蛋白酶分散的猴肾细胞悬液的制备与标准化
Proc Soc Exp Biol Med. 1954 Feb;85(2):202-5. doi: 10.3181/00379727-85-20830.
9
Plaque formation and isolation of pure lines with poliomyelitis viruses.脊髓灰质炎病毒的噬斑形成及纯系分离
J Exp Med. 1954 Feb;99(2):167-82. doi: 10.1084/jem.99.2.167.
10
Evaluation of 4-(2-hydroxyethyl)-1-piperazineëthanesulfonic acid (HEPES) as a tissue culture buffer.评估4-（2-羟乙基）-1-哌嗪乙磺酸（HEPES）作为组织培养缓冲液的性能。
Proc Soc Exp Biol Med. 1969 Jan;130(1):305-10. doi: 10.3181/00379727-130-33543.
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