一种体外转录的模板选择性抑制剂。
A template-selective inhibitor of in vitro transcription.
作者信息
Wilson D L, Geiduschek E P
出版信息
Proc Natl Acad Sci U S A. 1969 Feb;62(2):514-20. doi: 10.1073/pnas.62.2.514.
Abstract
A macromolecular factor, TF1, has been isolated from bacteriophage SP01-infected B. subtilis. TF1 selectively inhibits in vitro transcription of SP01 and related viral DNA. Evidence is presented regarding these properties of the repressor-like TF1: (1) template and conformational specificity; (2) interaction with DNA rather than RNA polymerase; (3) trypsin sensitivity; (4) reversibility of action; and (5) ability to block initiation, but not propagation, of RNA synthesis.
摘要
一种大分子因子TF1已从噬菌体SP01感染的枯草芽孢杆菌中分离出来。TF1能选择性地抑制SP01及相关病毒DNA的体外转录。本文提供了关于类阻遏物TF1这些特性的证据：（1）模板和构象特异性；（2）与DNA而非RNA聚合酶相互作用；（3）对胰蛋白酶敏感；（4）作用的可逆性；以及（5）阻断RNA合成起始而非延伸的能力。
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