Haselkorn R, Rouvière-Yaniv J
Proc Natl Acad Sci U S A. 1976 Jun;73(6):1917-20. doi: 10.1073/pnas.73.6.1917.
A DNA-binding protein has been isolated from two cyanobacteria (blue-green algae): Anabaena sp and Aphanocapsa sp. It has a molecular weight as determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis of about 10,000, is rich in lysine and arginine, and lacks tyrosine, tryptophan, and cysteine. The proteins from both strains show immunological identity with a similar DNA-binding protein from Escherichia coli, when tested by immunodiffusion with an antiserum prepared against the E. coli protein. Both the Aphanocapsa and E. coli proteins form compact, rapidly sedimenting complexes with cyanophage or bacteriophage DNA. The similarities between the proteins from cyanobacteria and E. coli suggest a degree of evolutionary conservation comparable to that of the histones of eukaryotes.
一种DNA结合蛋白已从两种蓝细菌（蓝绿藻）中分离出来：鱼腥藻属和隐球藻属。通过十二烷基硫酸钠聚丙烯酰胺凝胶电泳测定，其分子量约为10000，富含赖氨酸和精氨酸，且不含酪氨酸、色氨酸和半胱氨酸。当用针对大肠杆菌蛋白制备的抗血清进行免疫扩散试验时，这两种菌株的蛋白与来自大肠杆菌的一种类似DNA结合蛋白显示出免疫同一性。隐球藻属蛋白和大肠杆菌蛋白都能与蓝噬菌体或噬菌体DNA形成紧密的、快速沉降的复合物。蓝细菌和大肠杆菌蛋白之间的相似性表明，其进化保守程度与真核生物的组蛋白相当。
