1型马疱疹病毒包膜和核衣壳特异性抗体的病毒中和活性
The virus neutralizing activity of antibodies specific to the envelope and nucleocapsid of equine herpesvirus type 1.
作者信息
Papp-Vid G, Derbyshire J B
出版信息
Can J Comp Med. 1979 Apr;43(2):231-3.
Antibodies specific to the envelope or nucleocapsid of the Kentucky-D strain of equine herpesvirus type 1 were isolated from convalescent horse serum by immunoadsorption on cyanogen bromide-activated Sepharose conjugated with equine herpesvirus type 1 envelopes or nucleocapsids, with subsequent elution by glycine buffer. In double immunodiffusion and immunolectrophoresis reactions, the eluted proteins appeared to belong to the IgG fraction of horse serum. Antibodies directed against the viral envelope neutralized equine herpesvirus type 1 in a plaque neutralization test, while antibodies against the nucleocapsid showed no virus neutralizing activity.
通过在与1型马疱疹病毒包膜或核衣壳偶联的溴化氰活化琼脂糖上进行免疫吸附，随后用甘氨酸缓冲液洗脱，从康复马血清中分离出针对1型马疱疹病毒肯塔基-D株包膜或核衣壳的特异性抗体。在双向免疫扩散和免疫电泳反应中，洗脱的蛋白质似乎属于马血清的IgG组分。在蚀斑中和试验中，针对病毒包膜的抗体可中和1型马疱疹病毒，而针对核衣壳的抗体则无病毒中和活性。
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