肠毛细血管。II. 乙二胺四乙酸（EDTA）和组胺的结构效应。
Intestinal capillaries. II. Structural effects ofEDTA and histamine.
作者信息
Clementi F, Palade G E
出版信息
J Cell Biol. 1969 Sep;42(3):706-14. doi: 10.1083/jcb.42.3.706.
Abstract
Perfusion of the fenestrated capillaries of the intestinal mucosa of the rat with 0.05-0.1 M EDTA removes the diaphragms of the endothelial cells and detaches these cells from one another and from the basement membrane. The latter, even when completely denuded, retains effectively particles of 340 A (average) diameter. Perfusion with histamine (1 microg/ml) results in partial removal of fenestral diaphragms, occasional detachment of the endothelium from the basement membrane, and focal separation of endothelial intercellular junctions.
摘要
用0.05 - 0.1M的乙二胺四乙酸（EDTA）灌注大鼠肠黏膜的有孔毛细血管，可去除内皮细胞的隔膜，并使这些细胞相互分离以及与基底膜分离。即使基底膜完全裸露，它仍能有效地保留平均直径为340埃的颗粒。用组胺（1微克/毫升）灌注会导致有孔隔膜部分去除，内皮细胞偶尔与基底膜分离，以及内皮细胞间连接的局灶性分离。
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