将微生物脱氧核糖核酸分离成互补链。
Separation of microbial deoxyribonucleic acids into complementary strands.
作者信息
Rudner R, Karkas J D, Chargaff E
出版信息
Proc Natl Acad Sci U S A. 1969 May;63(1):152-9. doi: 10.1073/pnas.63.1.152.
Abstract
DNA preparations from seven bacterial species and from the E. coli phage T4 can, after denaturation with alkali, be separated chromatographically into two distinct components (L and H) through intermittent gradient elution from methylated albumin kieselguhr columns. The direct chemical analysis of the L and H fractions isolated from DNA specimens of the AT type shows them to exhibit a high degree of complementarity; but despite a bias in the distribution of purines and pyrimidines, either fraction contains equimolar quantities of 6-amino and of 6-keto nucleotides. In the L and H components derived from DNA of the equimolar and GC types, the distribution bias appears limited to guanine and cytosine. It is suggested that the L and H fractions represent the complementary DNA strands.
摘要
来自七种细菌物种以及大肠杆菌噬菌体T4的DNA制剂，在用碱变性后，通过从甲基化白蛋白硅藻土柱进行间歇梯度洗脱，可在色谱上分离成两个不同的组分（L和H）。对从AT型DNA标本中分离出的L和H组分进行直接化学分析表明，它们表现出高度的互补性；但是，尽管嘌呤和嘧啶的分布存在偏差，但任一馏分都含有等摩尔量的6-氨基和6-酮核苷酸。在来自等摩尔和GC型DNA的L和H组分中，分布偏差似乎仅限于鸟嘌呤和胞嘧啶。有人提出，L和H馏分代表互补的DNA链。
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