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天然和变性枯草芽孢杆菌DNA的RNA转录本的翻译互补性

Translational complementarity of RNA transcripts of native and denatured B. subtilis DNA.

作者信息

Cassuto E, Chargaff E

出版信息

Proc Natl Acad Sci U S A. 1970 Jul;66(3):863-8. doi: 10.1073/pnas.66.3.863.

Abstract

Previous evidence has suggested that the L and H fractions into which denatured DNA of B. subtilis can be separated by chromatography are complementary to each other with regard to base compositon, nucleotide sequence, and template properties, and that they may be regarded as families of fragments of the respective DNA strands. The present study tests the proposition that these fractions should also exhibit features of translational complementarity with respect to the DNA-dependent or the RNA-dependent incorporation of amino acids into ribosomes. This has been shown to be the case with the use of two pairs of amino acids: (1) lysine and phenylalanine; (2) proline and glycine.

摘要

先前的证据表明,枯草芽孢杆菌变性DNA经色谱法分离得到的L和H组分,在碱基组成、核苷酸序列和模板特性方面彼此互补,并且它们可被视为各自DNA链的片段家族。本研究检验了这样一个命题,即这些组分在氨基酸依赖DNA或依赖RNA掺入核糖体方面也应表现出翻译互补的特征。使用两对氨基酸已证明情况确实如此:(1)赖氨酸和苯丙氨酸;(2)脯氨酸和甘氨酸。

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