Stark D M, Norcross N L, Leahey J E
J Bacteriol. 1969 Aug;99(2):527-30. doi: 10.1128/jb.99.2.527-530.1969.
Rabbit and cow anti-Streptococcus dysgalactiae sera were tested by bacterial agglutination, complement fixation, hemagglutination, and immunodiffusion for the presence of antibody. The results of these tests were compared with mouse-protection studies on the same serum to estimate which in vitro test would best reflect the in vivo protective capacity of serum. Identification of the antibody constituents responsible for the mouse protection, hemagglutination, and complement fixation titers were established by reacting whole and diluted antisera with mercaptoethanol before and after testing. Results indicate that the complement fixation test may be a more accurate indicator of IgG protective bovine and rabbit antibody, whereas the hemagglutination test may more readily reflect a wider range of protective antibody levels and IgM. The complement fixation test showed some shared responses to IgG and IgM in both the rabbit and cow, whereas the IgM components seemed to be the predominant factor influencing hemagglutination titers in the rabbit and more so in the bovine. Mouse protection tests with mercaptoethanol-treated cow and rabbit sera indicate that the protective capacity of these antisera is shared between IgM and IgG components.
采用细菌凝集、补体结合、血凝和免疫扩散法检测兔和牛抗猪链球菌血清中的抗体。将这些检测结果与对同一血清进行的小鼠保护试验结果相比较，以评估哪种体外检测方法最能反映血清的体内保护能力。通过在检测前后用巯基乙醇处理全血清和稀释血清，确定了与小鼠保护、血凝和补体结合效价相关的抗体成分。结果表明，补体结合试验可能是IgG保护性牛和兔抗体的更准确指标，而血凝试验可能更容易反映更广泛的保护性抗体水平和IgM。补体结合试验在兔和牛中均显示出对IgG和IgM的一些共同反应，而IgM成分似乎是影响兔血凝效价的主要因素，对牛的影响更大。用巯基乙醇处理的牛和兔血清进行的小鼠保护试验表明，这些抗血清的保护能力由IgM和IgG成分共同发挥。
