Yoshida K, Ekstedt R D
J Bacteriol. 1968 Nov;96(5):1540-5. doi: 10.1128/jb.96.5.1540-1545.1968.
Antibody in hyperimmune rabbit antisera specific for Staphylococcus aureus teichoic acid was shown to be associated with the IgM fraction. Treatment of such sera with mercaptoethanol destroyed its activity in passive mouse protection tests, whereas absorption with antirabbit IgG had no effect. Antibody response in normal rabbits immunized by a single or by three daily injections of a killed vaccine of S. aureus was followed by a sensitive passive hemagglutination test. Antibody detected during the "primary" response was completely susceptible to reduction with mercaptoethanol. Most of the antibody detected after a secondary antigenic stimulation at 10 weeks was also susceptible to mercaptoethanol. The antibody titers correlated well with mouse protective activity, and this activity of the serum was also shown to be associated with the IgM fraction.
已证明，对金黄色葡萄球菌磷壁酸具有特异性的超免疫兔抗血清中的抗体与IgM组分相关。用巯基乙醇处理此类血清会破坏其在被动小鼠保护试验中的活性，而用抗兔IgG吸收则没有效果。通过单次或每日三次注射金黄色葡萄球菌灭活疫苗免疫的正常兔的抗体反应，随后通过灵敏的被动血凝试验进行监测。在“初次”反应期间检测到的抗体完全易被巯基乙醇还原。在10周时二次抗原刺激后检测到的大多数抗体也易被巯基乙醇作用。抗体滴度与小鼠保护活性密切相关，并且血清的这种活性也显示与IgM组分相关。
