Carlstedt-Duke J M, Elfström G, Högberg B, Gustafsson J A
Cancer Res. 1979 Nov;39(11):4653-6.
The ontogeny of the 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) receptor was studied in Sprague-Dawley rats by quantitation of the receptor in liver cytosol using isoelectric focusing in polyacrylamide gel. No differences by sex in the receptor concentration were seen at any of the ages studied. Newborn, 21-day-old, and 42-day-old rats contained significantly more receptor in the liver cytosol than did 56-day-old rats. There was no significant difference in the receptor concentration in liver cytosol from 7-day-old rats compared to that from 56-day-old rats. The maximum receptor concentration was found in cytosol from 21-day-old rats [36.1 +/- 24.0 (S.D.) fmol/mg protein]. Adult rats (56 days old) contained the lowest concentration of receptor (13.3 +/- 6.3 fmol/mg protein). The level of TCDD receptor in liver cytosol from adult rats was not significantly changed by orchiectomy, ovariectomy, adrenalectomy, or hypophysectomy. The maximum for TCDD receptor concentration at puberty corresponds to the reported maximum for the induction of aryl hydrocarbon hydroxylase activity. However, no further conclusion can as yet be drawn concerning the regulation of the TCDD receptor.
通过在聚丙烯酰胺凝胶中进行等电聚焦来定量肝胞质溶胶中的受体，研究了2,3,7,8-四氯二苯并对二恶英（TCDD）受体在斯普拉格-道利大鼠中的个体发育情况。在所研究的任何年龄阶段，均未观察到受体浓度存在性别差异。新生大鼠、21日龄大鼠和42日龄大鼠肝胞质溶胶中的受体含量明显高于56日龄大鼠。7日龄大鼠肝胞质溶胶中的受体浓度与56日龄大鼠相比无显著差异。在21日龄大鼠的胞质溶胶中发现了最高的受体浓度[36.1±24.0（标准差）fmol/mg蛋白质]。成年大鼠（56日龄）的受体浓度最低（13.3±6.3 fmol/mg蛋白质）。成年大鼠肝胞质溶胶中的TCDD受体水平在睾丸切除、卵巢切除、肾上腺切除或垂体切除后无显著变化。青春期TCDD受体浓度的最大值与报道的芳烃羟化酶活性诱导的最大值相对应。然而，关于TCDD受体的调节，目前尚无进一步结论。
