B组虫媒病毒库京病毒的蛋白质
Proteins of the group B arbovirus Kunjin.
作者信息
Westaway E G, Reedman B M
出版信息
J Virol. 1969 Nov;4(5):688-93. doi: 10.1128/JVI.4.5.688-693.1969.
Abstract
Purified Kunjin virus was disrupted with sodium dodecyl sulfate, urea, and mercaptoethanol or acetic acid. Electrophoresis on 7.5% polyacrylamide gel separated four proteins of high (120,000), intermediate (65,000) and low (18,000 and 13,000) molecular weights. A "core" particle was obtained by degradation of the virion with deoxycholate at 0 C; it contained the viral ribonucleic acid and the two small structural proteins. The "envelope" material released from around the core was identified with the most prominent (intermediate) protein seen in electropherograms of virion proteins. In addition to the structural proteins, at least three additional proteins (specified by virus infection) were found in the cytoplasm. The cytoplasmic proteins associated with Kunjin virus differed in their electrophoretic profile from those associated with infection by the related Murray Valley encephalitis virus.
摘要
纯化的库京病毒用十二烷基硫酸钠、尿素、巯基乙醇或乙酸进行裂解。在7.5%聚丙烯酰胺凝胶上进行电泳，分离出了分子量较高（120,000）、中等（65,000）以及较低（18,000和13,000）的四种蛋白质。在0℃下用脱氧胆酸盐降解病毒粒子可得到一种“核心”颗粒；它含有病毒核糖核酸和两种小结构蛋白。从核心周围释放出的“包膜”物质与病毒粒子蛋白质电泳图谱中最显著的（中等分子量）蛋白质一致。除了结构蛋白外，在细胞质中还发现了至少三种额外的蛋白质（由病毒感染产生）。与库京病毒相关的细胞质蛋白在电泳图谱上与相关的墨累谷脑炎病毒感染所产生的蛋白不同。
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2
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