Friedman R M
J Virol. 1968 Oct;2(10):1076-80. doi: 10.1128/JVI.2.10.1076-1080.1968.
Purified Semliki Forest virus (SFV) contains three structural proteins while its core (nucleocapsid) contains two of these proteins. To identify all of the proteins synthesized under virus direction, cells were infected with SFV in the presence of actinomycin D and guanidine. Cell protein synthesis was markedly and irreversibly inhibited under these conditions; virus growth was reversibly inhibited by guanidine and began when the cells were washed to remove the guanidine. When cells were treated with guanidine for 4 hr after virus infection and then were washed, five major proteins were produced early in infection. Three of these proteins corresponded to virus structural proteins. None of these five proteins was a major protein of uninfected cells or of virus-infected cells which had been incubated with partially purified interferon before infection. Late in infection, three major proteins, the virus structural proteins, were produced.
纯化的Semliki森林病毒（SFV）含有三种结构蛋白，而其核心（核衣壳）含有其中两种蛋白。为了鉴定在病毒指导下合成的所有蛋白质，细胞在放线菌素D和胍存在的情况下用SFV感染。在这些条件下，细胞蛋白质合成受到显著且不可逆的抑制；病毒生长受到胍的可逆抑制，当细胞洗涤以去除胍时开始生长。当细胞在病毒感染后用胍处理4小时然后洗涤时，在感染早期产生了五种主要蛋白质。其中三种蛋白质对应于病毒结构蛋白。这五种蛋白质中没有一种是未感染细胞或在感染前用部分纯化的干扰素孵育过的病毒感染细胞的主要蛋白质。在感染后期，产生了三种主要蛋白质，即病毒结构蛋白。
