厌氧菌磷酸解反应中辅酶A的需求
Requirement for coenzyme A in the phosphoroclastic reaction of anaerobic bacteria.
作者信息
Hespell R B, Joseph R, Mortlock R P
出版信息
J Bacteriol. 1969 Dec;100(3):1328-34. doi: 10.1128/jb.100.3.1328-1334.1969.
Abstract
Various bacteria which degrade pyruvate by the phosphoroclastic reaction were examined with respect to the role of coenzyme A (CoA) in this reaction. The strictly anaerobic bacteria, which cleave pyruvate by the phosphoroclastic reaction characteristic of Clostridia, required catalytic levels of CoA for the CO(2)-pyruvate exchange and acetoin-forming portions of the phosphoroclastic reaction. These reactions were reversibly inhibited by the CoA analogue, desulfo-CoA. In contrast, using cell-free extracts of bacteria which degrade pyruvate by the coliform phosphoroclastic reaction (pyruvate formate-lyase), no requirement for CoA could be observed for the formate-pyruvate exchange reaction. It is suggested that CoA serves a regulatory function in the early portion of the clostridal type of phosphoroclastic reaction.
摘要
就辅酶A（CoA）在磷酸解反应中所起的作用，对通过该反应降解丙酮酸的多种细菌进行了研究。严格厌氧的细菌通过梭菌属特有的磷酸解反应裂解丙酮酸，在磷酸解反应的CO₂ - 丙酮酸交换和生成乙偶姻部分需要催化量的CoA。这些反应被CoA类似物脱硫辅酶A可逆性抑制。相比之下，使用通过大肠菌磷酸解反应（丙酮酸甲酸裂解酶）降解丙酮酸的细菌的无细胞提取物，在甲酸 - 丙酮酸交换反应中未观察到对CoA的需求。有人提出，CoA在梭菌型磷酸解反应的早期部分起调节作用。
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1
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2
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J Bacteriol. 1962 Apr;83(4):887-98. doi: 10.1128/jb.83.4.887-898.1962.
3
Mechanisms of the formation of acetoin by yeast and mammalian tissue.酵母和哺乳动物组织生成乙偶姻的机制。
J Biol Chem. 1952 Apr;195(2):727-34.
4
Mechanisms of formation of acetoin by bacteria.细菌生成乙偶姻的机制。
J Biol Chem. 1952 Apr;195(2):715-26.
5
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J Biol Chem. 1951 Nov;193(1):265-75.
6
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J Biol Chem. 1951 Apr;189(2):815-30.
7
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Biochem Biophys Res Commun. 1965 Feb 3;18:303-7. doi: 10.1016/0006-291x(65)90703-5.
8
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