Hespell R B, Joseph R, Mortlock R P
J Bacteriol. 1969 Dec;100(3):1328-34. doi: 10.1128/jb.100.3.1328-1334.1969.
Various bacteria which degrade pyruvate by the phosphoroclastic reaction were examined with respect to the role of coenzyme A (CoA) in this reaction. The strictly anaerobic bacteria, which cleave pyruvate by the phosphoroclastic reaction characteristic of Clostridia, required catalytic levels of CoA for the CO(2)-pyruvate exchange and acetoin-forming portions of the phosphoroclastic reaction. These reactions were reversibly inhibited by the CoA analogue, desulfo-CoA. In contrast, using cell-free extracts of bacteria which degrade pyruvate by the coliform phosphoroclastic reaction (pyruvate formate-lyase), no requirement for CoA could be observed for the formate-pyruvate exchange reaction. It is suggested that CoA serves a regulatory function in the early portion of the clostridal type of phosphoroclastic reaction.
就辅酶A（CoA）在磷酸解反应中所起的作用，对通过该反应降解丙酮酸的多种细菌进行了研究。严格厌氧的细菌通过梭菌属特有的磷酸解反应裂解丙酮酸，在磷酸解反应的CO₂ - 丙酮酸交换和生成乙偶姻部分需要催化量的CoA。这些反应被CoA类似物脱硫辅酶A可逆性抑制。相比之下，使用通过大肠菌磷酸解反应（丙酮酸甲酸裂解酶）降解丙酮酸的细菌的无细胞提取物，在甲酸 - 丙酮酸交换反应中未观察到对CoA的需求。有人提出，CoA在梭菌型磷酸解反应的早期部分起调节作用。
