Hespell R B, Canale-Parola E
J Bacteriol. 1973 Nov;116(2):931-7. doi: 10.1128/jb.116.2.931-937.1973.
The pathways of glucose and pyruvate metabolism in Spirochaeta litoralis, a free-living, strictly anaerobic marine spirochete, were studied. Addition of 0.2 to 0.4 M NaCl (final concentration) to suspending buffers prevented cell lysis and was necessary for gas evolution from various substrates by cell suspensions. The organism fermented glucose mainly to ethanol, acetate, CO(2), and H(2). Determination of radioactivity in products formed from (14)C-labeled glucose and assays of enzymatic activities in cell extracts indicated that S. litoralis catabolized glucose via the Embden-Meyerhof pathway. A clostridial-type clastic reaction was utilized by the spirochete to degrade pyruvate to acetyl-coenzyme A, CO(2), and H(2). Formation of acetate from acetyl-coenzyme A was catalyzed by phosphotransacetylase and acetate kinase. Nicotinamide adenine dinucleotide-dependent acetaldehyde and alcohol dehydrogenases converted acetyl-coenzyme A to ethanol. A reversible hydrogenase activity was detected in cell extracts. S. litoralis cell extracts contained a rubredoxin similar in spectral properties to other bacterial rubredoxins.
对一种自由生活、严格厌氧的海洋螺旋体——海滨螺旋体（Spirochaeta litoralis）中的葡萄糖和丙酮酸代谢途径进行了研究。向悬浮缓冲液中添加0.2至0.4 M NaCl（终浓度）可防止细胞裂解，并且对于细胞悬液从各种底物中产生气体是必需的。该生物体将葡萄糖主要发酵为乙醇、乙酸盐、CO₂和H₂。对由¹⁴C标记的葡萄糖形成的产物中的放射性进行测定以及对细胞提取物中的酶活性进行测定表明，海滨螺旋体通过Embden-Meyerhof途径分解代谢葡萄糖。该螺旋体利用一种梭菌型裂解反应将丙酮酸降解为乙酰辅酶A、CO₂和H₂。磷酸转乙酰酶和乙酸激酶催化由乙酰辅酶A形成乙酸盐。烟酰胺腺嘌呤二核苷酸依赖性乙醛和乙醇脱氢酶将乙酰辅酶A转化为乙醇。在细胞提取物中检测到可逆的氢化酶活性。海滨螺旋体细胞提取物中含有一种铁氧化还原蛋白，其光谱特性与其他细菌铁氧化还原蛋白相似。
