Jameson J E
J Clin Pathol. 1969 Sep;22(5):515-8. doi: 10.1136/jcp.22.5.515.
Dilutions of a standard serum were made in saline and in negative sera, and comparative tests by immunodiffusion and by immunoosmophoresis were made against one antigenic component of Micropolyspora faeni (Thermopolyspora polyspora). The endpoint dilution of the standard serum in saline and serum in wells of different sizes was ascertained in each test. The endpoint dilution was unaffected by the distance of separation (from 1.5 mm to 6 mm) between the origins of serum and antigen, provided that the tests were read at times appropriate to each separation, the greater separations requiring longer periods of development. Higher endpoint dilutions were obtained by the use of 1% Noble agar in immunoosmophoresis than with 1% Ionagar no. 2 (Oxoid). This difference was more noticeable when low osmophoresis potentials were used.
将标准血清用生理盐水和阴性血清进行稀释，并针对嗜热放线多孢菌（嗜热多孢放线菌）的一种抗原成分，通过免疫扩散和免疫电泳进行对比试验。在每次试验中，确定标准血清在生理盐水以及不同大小孔中的血清中的终点稀释度。血清和抗原原点之间的分离距离（从1.5毫米到6毫米）不影响终点稀释度，前提是在适合每种分离距离的时间读取试验结果，分离距离越大，所需的显影时间越长。在免疫电泳中使用1%的Noble琼脂比使用1%的2号Ionagar（Oxoid）可获得更高的终点稀释度。当使用低电泳电位时，这种差异更为明显。
