豚鼠反应素抗体。II. 物理化学和生物学特性。
Guinea-pig reaginic antibody. II. Physicochemical and biological properties.
作者信息
Margni R A, Hajos S E
出版信息
Immunology. 1973 Aug;25(2):333-42.
The physicochemical and biological properties of purified guinea-pig reaginic antibody were studied. It is a labile protein different to γ. Its antibody activity is completely destroyed by heating at 56° for 6 hours and by treatment with mercaptoethanol. The capacity to give PCA is decreased by repeated freezing and thawing. It is a bivalent antibody, haemagglutinating, does not fix complement and is capable of sensitizing guinea-pig skin for PCA reaction after a latent period of a week but not after 3 hours. Reaginic antibody appears on day 7–8 after the first inoculation and the higher levels (PCA reaction) are obtained at the eleventh to thirteenth days. After the fifteenth to seventeenth days the PCA is negative. The reaginic antibody does not pass the placenta. Higher levels of reaginic antibody were obtained when the guinea-pigs were inoculated with the antigen in saline with simultaneous inoculation, intraperitoneally, of killed , phase I.
对纯化的豚鼠反应素抗体的理化和生物学特性进行了研究。它是一种与γ球蛋白不同的不稳定蛋白质。其抗体活性在56℃加热6小时或用巯基乙醇处理后会完全被破坏。反复冻融会降低其产生被动皮肤过敏反应（PCA）的能力。它是一种二价抗体，具有血凝作用，不固定补体，在一周的潜伏期后能够使豚鼠皮肤对PCA反应敏感，但3小时后则不能。反应素抗体在首次接种后第7 - 8天出现，在第11至13天达到较高水平（PCA反应）。第15至17天后PCA呈阴性。反应素抗体不能通过胎盘。当豚鼠同时腹腔注射用生理盐水稀释的抗原以及I期死菌时，可获得更高水平的反应素抗体。
Zotero 插件