Somerville H J, Delafield F P, Rittenberg S C
J Bacteriol. 1970 Feb;101(2):551-60. doi: 10.1128/jb.101.2.551-560.1970.
Treatment with urea-mercaptoethanol of purified spores of Bacillus thuringiensis, other Bacillus species, and Clostridium roseum solubilizes a protein fraction between 5 and 12% of the dry weight of the spores. This fraction behaves identically to the crystal protein of B. thuringiensis on acrylamide-gel electrophoresis. The protein from all of the Bacillus species shows partial homology with crystal protein, using the Ouchterlony immunodiffusion technique. A further fraction, similar in amount, can be removed from spores of B. thuringiensis by the addition of sodium lauryl sulfate to the urea-mercaptoethanol. Spores of B. thuringiensis extracted in these ways show no difference when compared to untreated spores with respect to viability or resistance to heat and ultraviolet-irradiation. The extracted spores do show differences in their germination requirements and their susceptibility to phase-darkening by lysozyme. It is concluded that an urea-mercaptoethanol-soluble protein or class of protein is a widespread component of bacterial spores, possibly located in the spore coat, and that this protein may be related to the crystal protein of B. thuringiensis.
用尿素 - 巯基乙醇处理苏云金芽孢杆菌、其他芽孢杆菌属以及玫瑰色梭菌的纯化孢子，可溶解孢子干重5%至12%的蛋白质部分。该部分在丙烯酰胺凝胶电泳上的行为与苏云金芽孢杆菌的晶体蛋白相同。使用奥克特洛尼免疫扩散技术，所有芽孢杆菌属的蛋白质都与晶体蛋白显示出部分同源性。通过向尿素 - 巯基乙醇中添加十二烷基硫酸钠，可从苏云金芽孢杆菌的孢子中去除另一部分数量相似的蛋白质。以这些方式提取的苏云金芽孢杆菌孢子与未处理的孢子相比，在活力或对热和紫外线辐射的抗性方面没有差异。提取的孢子在萌发要求和对溶菌酶导致的相暗化敏感性方面确实存在差异。得出的结论是，尿素 - 巯基乙醇可溶性蛋白质或一类蛋白质是细菌孢子的广泛组成成分，可能位于孢子壁中，并且这种蛋白质可能与苏云金芽孢杆菌的晶体蛋白有关。
