Rose N R, Stylos W A
Clin Exp Immunol. 1969 Jul;5(1):129-40.
Human thyroglobulin was purified by ammonium sulphate precipitation followed by DEAE column chromatography. It produced a single line in double diffusion precipitation reactions in gel and immunoelectrophoresis, and was devoid of serum-related antigens. The preparation was reduced by 2-mercaptoethanol and alkylated by iodoacetamide in the presence and the absence of 8 M-urea. In the absence of urea, the reduced, alkylated product was still capable of inhibiting the tanned cell haemagglutinin reaction of human autoantiserum or rabbit heteroantiserum with native thyroglobulin. The rabbit, but not the human, antiserum precipitated reduced, alkylated thyroglobulin in agar gel. By immunoelectrophoresis, three antigens migrating faster than thyroglobulin were seen with the rabbit antiserum. The same preparation, reduced and alkylated in the presence of 8 M-urea, failed to react with either the rabbit or human antisera.
人甲状腺球蛋白通过硫酸铵沉淀，然后进行DEAE柱层析进行纯化。它在凝胶双扩散沉淀反应和免疫电泳中产生单一沉淀线，并且不含血清相关抗原。该制剂在有和没有8M尿素存在的情况下，用2-巯基乙醇还原并用碘乙酰胺烷基化。在没有尿素的情况下，还原烷基化产物仍然能够抑制人自身抗血清或兔异种抗血清与天然甲状腺球蛋白的鞣酸细胞血凝反应。兔抗血清而非人抗血清在琼脂凝胶中沉淀还原烷基化的甲状腺球蛋白。通过免疫电泳，用兔抗血清可观察到三种迁移速度比甲状腺球蛋白快的抗原。在8M尿素存在下还原烷基化的相同制剂，不能与兔或人抗血清发生反应。
