Stranks D W, Bieniada J
Mycopathologia. 1975 Feb 28;55(1):57-63. doi: 10.1007/BF00467093.
Cellulase can be produced from growth in noncellulosic substrate if the growth rate of the producing organism is restricted. Phenethyl alcohol (PEA) is a growth inhibitor and was used to control the growth of M. verrucaria in attempts to obtain increased cellulase production. Cellulase yield was found to be increased without a restriction in growth rate when PEA was present in low concentrations (0.03% v/v). The effect was observed for other organisms but notably L. trabea, which produced considerable enzyme from a small quantity of mycelium. Here increased cellulase synthesis was concomitant with restricted growth. Other chemicals with PEA-like structure (e.g. benzyl alcohol) resulted in similar or more extensive cellulase synthesis. Of the substances tried, propyl alcohol was most effective, followed by acetone. PEA causes a swelling of cell walls and inhibits spore formation. This and other data given suggest that PEA affects the cytoplasmic membrane or the cell wall or both. Cellulase synthesis is considered to take place in the membrane and wall region of the cell.
如果产酶生物的生长速率受到限制，纤维素酶可以在非纤维素底物中生长产生。苯乙醇（PEA）是一种生长抑制剂，曾被用于控制疣孢漆斑菌的生长，试图提高纤维素酶的产量。当低浓度（0.03% v/v）的PEA存在时，发现纤维素酶产量增加且生长速率不受限制。在其他生物中也观察到了这种效应，尤其是束状炭角菌，它能从少量菌丝体中产生大量酶。在这里，纤维素酶合成增加与生长受限同时出现。具有类似PEA结构的其他化学物质（如苯甲醇）导致了相似或更广泛的纤维素酶合成。在所试验的物质中，丙醇最有效，其次是丙酮。PEA会导致细胞壁肿胀并抑制孢子形成。给出的这些及其他数据表明，PEA会影响细胞质膜或细胞壁或两者。纤维素酶合成被认为发生在细胞的膜和壁区域。
