TREICK R W, KONETZKA W A
J Bacteriol. 1964 Dec;88(6):1580-4. doi: 10.1128/jb.88.6.1580-1584.1964.
Treick, R. W. (Indiana University, Bloomington), and W. A. Konetzka. Physiological state of Escherichia coli and the inhibition of deoxyribonucleic acid synthesis by phenethyl alcohol. J. Bacteriol. 88:1580-1584. 1964.-The effects of concentration of phenethyl alcohol (PEA) and the physiological state of the cells on inhibition of macromolecular synthesis in Escherichia coli were investigated. Deoxyribonucleic acid (DNA) synthesis by cells of E. coli from the maximum stationary phase is completely inhibited by 0.32% (v/v) PEA immediately upon addition of the inhibitor, although there is a net increase in the synthesis of ribonucleic acid (RNA) and protein. However, DNA synthesis in cells from the exponential phase is inhibited only after an increase which corresponds to 1.4 to 1.6 times the amount of DNA present at the time of PEA addition. In a randomly dividing culture, this increment of DNA synthesis presumably represents completion of the DNA replication cycle initiated at the time of PEA addition. By programming the addition and removal of PEA, DNA synthesis can be made to proceed in stepwise increments corresponding to doublings of the DNA. The data indicate that the DNA being replicated at the time of PEA addition completes the replication cycle and, although there is net synthesis of RNA and protein, no initiation of a second cycle of DNA replication occurs until the removal of the inhibitor.
特雷里克，R.W.（印第安纳大学，布卢明顿分校），以及W.A.科内茨卡。大肠杆菌的生理状态及苯乙醇对脱氧核糖核酸合成的抑制作用。《细菌学杂志》88:1580 - 1584。1964年。——研究了苯乙醇（PEA）浓度和细胞生理状态对大肠杆菌大分子合成抑制作用的影响。来自最大稳定期的大肠杆菌细胞的脱氧核糖核酸（DNA）合成在加入抑制剂后立即被0.32%（v/v）的PEA完全抑制，尽管核糖核酸（RNA）和蛋白质的合成有净增加。然而，指数期细胞中的DNA合成仅在增加量相当于加入PEA时存在的DNA量的1.4至1.6倍之后才被抑制。在随机分裂的培养物中，这种DNA合成的增加量大概代表了在加入PEA时开始的DNA复制周期的完成。通过对PEA的添加和去除进行编程，DNA合成可以以与DNA加倍相对应的逐步增量进行。数据表明，在加入PEA时正在复制的DNA完成了复制周期，并且尽管有RNA和蛋白质的净合成，但在去除抑制剂之前不会开始第二个DNA复制周期。
