免疫固定法与交叉免疫电泳法在检测C3激活产物方面的比较。

Comparison between immunofixation and crossed immunoelectrophoresis for the detection of C3 activation products.

作者信息

De Vecchi A, Montagnino G, Massaro L, Constantino A, Bencini P L, Tarantino A

出版信息

Clin Chim Acta. 1979 Sep 15;97(1):27-32. doi: 10.1016/0009-8981(79)90021-4.

Abstract

C3 breakdown products were measured in 51 fresh and stored sera and/or EDTA plasma samples from 18 healthy subjects, 8 patients affected by essential mixed cryoglobulinaemia, and 15 patients with miscellaneous glomerulonephritis, by simultaneous crossed immunoelectrophoresis and immunofixation. C3 splitting products, as determined by both methods compared well, and showed a highly significant correlation. The advantages and reliability of these two methods are discussed. Immunofixation seems to be the most suitable for routine use in clinical practice, being less expensive and more rapid to perform.

摘要

通过同步交叉免疫电泳和免疫固定法，对18名健康受试者、8名患有原发性混合性冷球蛋白血症的患者以及15名患有其他类型肾小球肾炎的患者的51份新鲜和储存的血清及/或乙二胺四乙酸血浆样本中的C3裂解产物进行了测量。两种方法测定的C3裂解产物结果比较吻合，且显示出高度显著的相关性。讨论了这两种方法的优点和可靠性。免疫固定法似乎最适合临床实践中的常规使用，因为其成本较低且操作更快速。