Brandslund I, Siersted H C, Svehag S E, Teisner B
J Immunol Methods. 1981;44(1):63-71. doi: 10.1016/0022-1759(81)90107-1.
A double-decker rocket immunoelectrophoresis (DD-RIE) method for direct quantitation of complement split products with C3d determinants in human plasma is described. The usefulness of the DD-RIE method for monitoring C3 activation has been assessed and compared with conventional crossed immunoelectrophoresis (CIE) for C3c determination in a patient with iatrogenic septic shock and patients with rheumatoid arthritis. In contrast with CIE the DD-RIE method is quantitative by reference to a standard curve based on an internal reference C3d preparation and its sensitivity and assay capacity are superior to CIE. All reagents and antibody preparation are commercially available and the production of standards is easy. No overlapping was observed between C3d values in plasma from healthy persons and patients with active classical rheumatoid arthritis. The DD-RIE is highly suitable for routine use in laboratories of clinical immunology.
本文描述了一种用于直接定量人血浆中具有C3d决定簇的补体裂解产物的双层火箭免疫电泳（DD-RIE）方法。已评估了DD-RIE方法监测C3活化的实用性，并与传统交叉免疫电泳（CIE）在一名医源性脓毒症休克患者和类风湿性关节炎患者中测定C3c的方法进行了比较。与CIE不同，DD-RIE方法通过基于内部参考C3d制剂的标准曲线进行定量，其灵敏度和检测能力优于CIE。所有试剂和抗体制剂均可商购，标准品的制备也很容易。在健康人和活动性典型类风湿性关节炎患者的血浆中未观察到C3d值有重叠。DD-RIE非常适合临床免疫实验室的常规使用。
