一种用于定量生物体液中补体C3c的单步免疫电泳方法。

A single step immunoelectrophoresis method for the quantitation of complement C3c in biological fluids.

作者信息

Berkowicz A, Rosenkvist J, Sørensen H

出版信息

J Immunol Methods. 1983 Jun 10;60(3):277-88. doi: 10.1016/0022-1759(83)90286-7.

Abstract

An intermediate gel rocket immunoelectrophoresis (IRI) suitable for direct quantitation of complement split products with C3c specificity is described. The technique represents a simple, quick, and reproducible method for the assessment of complement activity in biological fluids in diseases. The advantages of the quantitative approach of the IRI method as compared to the conventional crossed immunoelectrophoresis (CIE) are the increased sensitivity, specificity, and precision of the method; also, the assay capacity is superior to CIE. The IRI-C3c assay is therefore highly suitable as a scientific tool, and may also be useful for routine laboratory investigations.

摘要

本文描述了一种适用于直接定量具有C3c特异性补体裂解产物的中间凝胶火箭免疫电泳（IRI）。该技术是一种简单、快速且可重复的方法，用于评估疾病生物体液中的补体活性。与传统交叉免疫电泳（CIE）相比，IRI方法定量方法的优点是该方法的灵敏度、特异性和精密度提高；此外，检测能力优于CIE。因此，IRI - C3c检测非常适合作为一种科学工具，也可能有助于常规实验室研究。