Crofton P M, Smith A F
Clin Chim Acta. 1979 Nov 2;98(3):253-61. doi: 10.1016/0009-8981(79)90152-9.
An ion-exchange method for the measurement of a high molecular weight form of alkaline phosphatase in serum is described. The method is simple, rapid, precise and suitable for processing small batches of samples. Other forms of alkaline phosphatase commonly encountered in serum do not interfere. The correlation between results obtained by this method and those obtained by Sephadex 6B chromatography is discussed. Electrophoretic methods of measurement were also investigated but were found to be both imprecise and inaccurate.
本文描述了一种用于测量血清中高分子量碱性磷酸酶的离子交换法。该方法简单、快速、精确,适用于处理小批量样本。血清中常见的其他形式的碱性磷酸酶不会产生干扰。讨论了该方法与通过葡聚糖凝胶6B色谱法获得的结果之间的相关性。还研究了电泳测量方法,但发现其既不精确也不准确。