Weltzien H U, Jesaitis M A
J Exp Med. 1971 Mar 1;133(3):534-53. doi: 10.1084/jem.133.3.534.
Cell walls of E. coli strains B and Cullen contain specific receptors for colicin K and for the T2, T6, and C16 phages. The receptors for the bacteriocin and the T6 virus are located in the outer layers of the cell wall of these microorganisms and are absent in their cytoplasmic membrane. The receptors for colicin K, phage T2, and the T6 and C16 viruses differ in their stability toward enzymes and chemical reagents. Their specificity must therefore be determined by different chemical groupings. The colicin K receptor is inactivated by certain proteolytic enzymes and by reagents which combine with tryptophan. It is concluded therefore that proteins or peptides containing this amino acid are essential for biological activity of the receptor.
大肠杆菌B株和卡伦株的细胞壁含有针对大肠杆菌素K以及T2、T6和C16噬菌体的特异性受体。细菌素和T6病毒的受体位于这些微生物细胞壁的外层,而在它们的细胞质膜中不存在。大肠杆菌素K、噬菌体T2以及T6和C16病毒的受体对酶和化学试剂的稳定性不同。因此,它们的特异性必定由不同的化学基团决定。大肠杆菌素K受体可被某些蛋白水解酶以及与色氨酸结合的试剂所灭活。因此可以得出结论,含有这种氨基酸的蛋白质或肽对于受体的生物活性至关重要。