专性厌氧菌巨大脱硫弧菌中原卟啉原的氧化作用。
Oxidation of protoporphyrinogen in the obligate anaerobe Desulfovibrio gigas.
作者信息
Klemm D J, Barton L L
出版信息
J Bacteriol. 1985 Oct;164(1):316-20. doi: 10.1128/jb.164.1.316-320.1985.
Abstract
The anaerobic oxidation of protoporphyrinogen to protoporphyrin was demonstrated in extracts of Desulfovibrio gigas. Protoporphyrin formation occurred in the presence of nitrite, hydroxylamine, sulfite, thiosulfate, ATP plus sulfate, NAD+, NADP+, flavin adenine dinucleotide, flavin mononucleotide, fumarate, 2,6-dichlorophenol-indophenol, methyl viologen, and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide. With dialyzed cell extracts, highest activities were observed with sulfite, NAD+, and NADP+ as electron acceptors. The enzyme for protoporphyrinogen oxidation was localized in the membrane of D. gigas and displayed optimal activity at pH 7.3 and 28 degrees C.
摘要
在巨大脱硫弧菌提取物中证实了原卟啉原厌氧氧化为原卟啉的过程。原卟啉的形成发生在亚硝酸盐、羟胺、亚硫酸盐、硫代硫酸盐、ATP加硫酸盐、NAD⁺、NADP⁺、黄素腺嘌呤二核苷酸、黄素单核苷酸、富马酸盐、2,6 - 二氯酚靛酚、甲基紫精和3 -（4,5 - 二甲基噻唑 - 2 - 基）- 2,5 - 二苯基溴化四氮唑存在的情况下。对于透析后的细胞提取物，以亚硫酸盐、NAD⁺和NADP⁺作为电子受体时观察到最高活性。原卟啉原氧化酶定位于巨大脱硫弧菌的膜中，在pH 7.3和28℃时表现出最佳活性。
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