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ENZYMATIC SYNTHESIS OF DEOXYRIBONUCLEIC ACID. IV. LINKAGE OF SINGLE DEOXYNUCLEOTIDES TO THE DEOXYNUCLEOSIDE ENDS OF DEOXYRIBONUCLEIC ACID.脱氧核糖核酸的酶促合成。IV. 单脱氧核苷酸与脱氧核糖核酸脱氧核苷末端的连接
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THE USE OF PURIFIED MICROCOCCAL NUCLEASE IN IDENTIFYING THE NUCLEOTIDE TERMINUS BEARING A FREE 5'-MONOPHOSPHATE.纯化的微球菌核酸酶在鉴定带有游离5'-单磷酸的核苷酸末端中的应用。
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THE DEOXYRIBONUCLEASES OF ESCHERICHIA COLI. V. ON THE SPECIFICITY OF EXONUCLEASE I (PHOSPHODIESTERASE).大肠杆菌的脱氧核糖核酸酶。V. 关于核酸外切酶I（磷酸二酯酶）的特异性
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ENZYMIC SYNTHESIS OF DEOXYRIBONUCLEIC ACID. 18. THE REPAIR OF PARTIALLY SINGLE-STRANDED DNA TEMPLATES BY DNA POLYMERASE.脱氧核糖核酸的酶促合成。18. 用DNA聚合酶修复部分单链DNA模板
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DEOXYTHYMIDINE KINASE OF ESCHERICHIA COLI. I. PURIFICATION AND SOME PROPERTIES OF THE ENZYME.大肠杆菌的脱氧胸苷激酶。I. 酶的纯化及某些性质
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ENZYMATIC SYNTHESIS OF DEOXYRIBONUCLEIC ACID. XV. PURIFICATION AND PROPERTIES OF A POLYMERASE FROM BACILLUS SUBTILIS.脱氧核糖核酸的酶促合成。十五。枯草芽孢杆菌中一种聚合酶的纯化及性质
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A DEOXYRIBONUCLEIC ACID PHOSPHATASE-EXONUCLEASE FROM ESCHERICHIA COLI. I. PURIFICATION OF THE ENZYME AND CHARACTERIZATION OF THE PHOSPHATASE ACTIVITY.来自大肠杆菌的一种脱氧核糖核酸磷酸酶-核酸外切酶。I. 酶的纯化及磷酸酶活性的表征
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A membrane-filter technique for the detection of complementary DNA.一种用于检测互补DNA的膜过滤技术。
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Polydeoxynucleotide intermediates in DNA replication in regenerating liver.再生肝脏中DNA复制过程中的多脱氧核苷酸中间体
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DNA链生长的机制。IV。通过核酸外切酶降解揭示的T4短DNA链的合成方向。

Mechanism of DNA chain growth. IV. Direction of synthesis of T4 short DNA chains as revealed by exonucleolytic degradation.

作者信息

Okazaki T, Okazaki R

出版信息

Proc Natl Acad Sci U S A. 1969 Dec;64(4):1242-8. doi: 10.1073/pnas.64.4.1242.

DOI:10.1073/pnas.64.4.1242
PMID:4989398
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC223275/
Abstract

T4 nascent short chains labeled at their growing ends with H(3)-thymidine and uniformly with C(14)-thymidine were prepared, separated into complementary strands, and degraded by E. coli exonuclease I in the 3' to 5' direction or by B. subtilis nuclease in the 5' to 3' direction. The kinetics of release of H(3) and C(14) labels by both enzymes was consistent with the conclusion that the H(3) label is at the 3' end of the nascent short chains of both strands and that the short chains are products of discontinuous synthesis in the 5' to 3' direction along the two template strands.

摘要

制备了在其生长末端用H(3)-胸腺嘧啶核苷标记且均匀用C(14)-胸腺嘧啶核苷标记的T4新生短链，将其分离成互补链，并在3'至5'方向上用大肠杆菌核酸外切酶I或在5'至3'方向上用枯草芽孢杆菌核酸酶进行降解。两种酶释放H(3)和C(14)标记的动力学与以下结论一致：H(3)标记位于两条链新生短链的3'末端，并且短链是沿着两条模板链在5'至3'方向上不连续合成的产物。