DNA链生长的机制。IV。通过核酸外切酶降解揭示的T4短DNA链的合成方向。
Mechanism of DNA chain growth. IV. Direction of synthesis of T4 short DNA chains as revealed by exonucleolytic degradation.
作者信息
Okazaki T, Okazaki R
出版信息
Proc Natl Acad Sci U S A. 1969 Dec;64(4):1242-8. doi: 10.1073/pnas.64.4.1242.
Abstract
T4 nascent short chains labeled at their growing ends with H(3)-thymidine and uniformly with C(14)-thymidine were prepared, separated into complementary strands, and degraded by E. coli exonuclease I in the 3' to 5' direction or by B. subtilis nuclease in the 5' to 3' direction. The kinetics of release of H(3) and C(14) labels by both enzymes was consistent with the conclusion that the H(3) label is at the 3' end of the nascent short chains of both strands and that the short chains are products of discontinuous synthesis in the 5' to 3' direction along the two template strands.
摘要
制备了在其生长末端用H(3)-胸腺嘧啶核苷标记且均匀用C(14)-胸腺嘧啶核苷标记的T4新生短链，将其分离成互补链，并在3'至5'方向上用大肠杆菌核酸外切酶I或在5'至3'方向上用枯草芽孢杆菌核酸酶进行降解。两种酶释放H(3)和C(14)标记的动力学与以下结论一致：H(3)标记位于两条链新生短链的3'末端，并且短链是沿着两条模板链在5'至3'方向上不连续合成的产物。
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