DNA链生长机制。3. T4新生短DNA链与噬菌体DNA分离的互补链的均等退火。
Mechanism of DNA chain growth. 3. Equal annealing of T4 nascent short DNA chains with the separated complementary strands of the phage DNA.
作者信息
Sugimoto K, Okazaki T, Imae Y, Okazaki R
出版信息
Proc Natl Acad Sci U S A. 1969 Aug;63(4):1343-50. doi: 10.1073/pnas.63.4.1343.
Abstract
Nascent short DNA chains isolated from T4-infected E. coli under a variety of conditions anneal equally to the separated complementary phage DNA strands. The samples examined include: pulse-labeled short chains isolated by alkaline sucrose gradient sedimentation from the T4D (wild type)-infected cells in both the early and late stages of phage DNA synthesis; nascent chains accumulated during ligase inhibition of T4 ts B20-infected cells; and the single-stranded nascent short chains isolated from T4D-infected cells by mild procedures involving no denaturation treatment. The results are consistent with the hypothesis that both strands of DNA are synthesized discontinuously.
摘要
在各种条件下从T4感染的大肠杆菌中分离出的新生短DNA链，与分离的互补噬菌体DNA链的退火情况相同。所检测的样本包括：通过碱性蔗糖梯度沉降从噬菌体DNA合成早期和晚期的T4D（野生型）感染细胞中分离出的脉冲标记短链；T4 ts B20感染细胞在连接酶抑制期间积累的新生链；以及通过不涉及变性处理的温和程序从T4D感染细胞中分离出的单链新生短链。结果与DNA的两条链都是不连续合成的假说一致。
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