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感染风疹病毒的猴肾细胞培养物的电子显微镜检查。

Electron microscopy of monkey kidney cell cultures infected with rubella virus.

作者信息

Kim K S, Boatman E S

出版信息

J Virol. 1967 Feb;1(1):205-14. doi: 10.1128/JVI.1.1.205-214.1967.

DOI:10.1128/JVI.1.1.205-214.1967
PMID:4990037
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC375520/
Abstract

Two rubella virus strains isolated in this laboratory were investigated in terms of their growth in LLC-MK(2) cell cultures and their effect on cell morphology. Rubella virus grew readily in LLC-MK(2) cells, but cytopathic effects of the virus were not observed in infected cultures. Such infected cultures can be subcultured indefinitely and continue to shed virus. Examination of rubella-infected cell cultures by electron microscopy showed the presence of annulate lamellae in the cytoplasm of 15% of the cells. No changes were evident in the nuclei. These membranous inclusions varied in complexity from parallel arrays of annulate lamellae to large lamellar structures of complex morphology. An occasional cell contained a crystal lattice structure in association with the lamellae. Larger inclusions, consisting of disorganized arrays of "unit" membranes, were also found. Uninfected cells were devoid of annulate lamellae, crystals, and complex membranous inclusions. No viruslike particles were observed in any part of the cells from infected cultures. The significance of the structures observed has not been determined.

摘要

对本实验室分离出的两株风疹病毒进行了研究，观察它们在LLC-MK(2)细胞培养物中的生长情况及其对细胞形态的影响。风疹病毒在LLC-MK(2)细胞中易于生长，但在感染的培养物中未观察到病毒的细胞病变效应。此类感染的培养物可无限传代并持续释放病毒。通过电子显微镜检查风疹感染的细胞培养物发现，15%的细胞胞质中存在环状片层。细胞核未见明显变化。这些膜性内含物的复杂程度各不相同，从平行排列的环状片层到形态复杂的大的片层结构。偶尔有细胞含有与片层相关的晶格结构。还发现了由“单位”膜无序排列组成的较大内含物。未感染的细胞没有环状片层、晶体和复杂的膜性内含物。在感染培养物的细胞任何部位均未观察到病毒样颗粒。所观察到的结构的意义尚未确定。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7d3/375520/35f3a58ceecb/jvirol00325-0222-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7d3/375520/d6eb74b84556/jvirol00325-0216-a.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7d3/375520/81b79f8536a4/jvirol00325-0218-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7d3/375520/57e3ae5fec48/jvirol00325-0219-a.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7d3/375520/7bdb4ddf1cba/jvirol00325-0221-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7d3/375520/6d0d241bcc6c/jvirol00325-0221-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7d3/375520/35f3a58ceecb/jvirol00325-0222-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7d3/375520/d6eb74b84556/jvirol00325-0216-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7d3/375520/6c64980a359c/jvirol00325-0216-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7d3/375520/e94709d9f139/jvirol00325-0218-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7d3/375520/81b79f8536a4/jvirol00325-0218-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7d3/375520/57e3ae5fec48/jvirol00325-0219-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7d3/375520/821de21c54d0/jvirol00325-0220-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7d3/375520/8e32ea446f81/jvirol00325-0220-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7d3/375520/7bdb4ddf1cba/jvirol00325-0221-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7d3/375520/6d0d241bcc6c/jvirol00325-0221-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7d3/375520/35f3a58ceecb/jvirol00325-0222-a.jpg

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