Messner R P, Jelinek J
J Clin Invest. 1970 Dec;49(12):2165-71. doi: 10.1172/JCI106435.
Cell surface receptors for human gammaG antibodies directed against bacterial antigens were demonstrated on human neutrophils using an in vitro bacteriocidal-phagocytic assay. These results were confirmed by adherence of sensitized erythrocytes to monolayers of neutrophils or monocytes. Erythrocytes sensitized indirectly with antibacterial gammaG antibodies after passive sensitization with bacterial antigens adhered to both neutrophils and monocytes. Erythrocytes sensitized directly with conventional anti-D gammaG antibodies adhered only to monocytes, while those sensitized with the hyperimmune anti-CD gammaG antibody Ripley adhered to both monocytes and neutrophils. Adherence of anti-Rh or antibacterial gammaG antibodies to monocytes and neutrophils could be inhibited by whole gammaG, myeloma globulins of the gamma(1) or gamma(3) subclasses, or Fc fragments, but not by Fab fragment. These results indicate that receptors for the Fc portion of human gammaG antibodies exist on both neutrophils and monocytes, and that gammaG antibodies differ in their ability to attach to these two cell types. Differences in the behavior of the gammaG antibodies studied may be related to differences in the density of antibodies on the erythrocyte surface and receptors on the phagocytic cells.
利用体外杀菌吞噬试验在人中性粒细胞上证实了针对细菌抗原的人γG抗体的细胞表面受体。通过致敏红细胞与中性粒细胞或单核细胞单层的黏附，证实了这些结果。在用细菌抗原被动致敏后用抗菌γG抗体间接致敏的红细胞，能黏附于中性粒细胞和单核细胞。用常规抗-DγG抗体直接致敏的红细胞仅黏附于单核细胞，而用超免疫抗-CDγG抗体里普利致敏的红细胞则黏附于单核细胞和中性粒细胞。抗-Rh或抗菌γG抗体与单核细胞和中性粒细胞的黏附可被完整的γG、γ(1)或γ(3)亚类的骨髓瘤球蛋白或Fc片段抑制，但不能被Fab片段抑制。这些结果表明，人γG抗体Fc部分的受体存在于中性粒细胞和单核细胞上，并且γG抗体在附着于这两种细胞类型的能力上存在差异。所研究的γG抗体行为的差异可能与红细胞表面抗体密度和吞噬细胞上受体的差异有关。
