Waites W M, Kay D, Dawes I W, Wood D A, Warren S C, Mandelstam J
Biochem J. 1970 Jul;118(4):667-76. doi: 10.1042/bj1180667.
A comparison was made of morphological changes and successive, mainly biochemical, marker events for sporulation in 14 asporogenous mutants. The morphological and biochemical sequences are linked so that arrested development in one is accompanied by corresponding effects in the other. Thus mutants that fail to produce both protease and antibiotic do not progress beyond stage 0, formation of alkaline phosphatase appears to be associated with the transition from stage II to stage III and glucose dehydrogenase with that from stage III to stage IV. Stage II mutants may produce ;pygmy' cells or other bizarre cell-division forms. The biochemical sequence is dependent in the sense that if the occurrence of any one event is blocked that of all the succeeding events is also blocked. This has implications for biochemical models that have been proposed to explain the temporal sequence observed in spore development.
对14个无孢子形成突变体中孢子形成的形态变化以及连续的、主要是生化方面的标记事件进行了比较。形态和生化序列是相关联的，因此一个方面的发育停滞会伴随另一个方面产生相应的影响。所以，既不能产生蛋白酶也不能产生抗生素的突变体不会超过0期，碱性磷酸酶的出现似乎与从II期到III期的转变相关，而葡萄糖脱氢酶则与从III期到IV期的转变相关。II期突变体可能会产生“侏儒”细胞或其他奇异的细胞分裂形式。生化序列具有依赖性，即如果任何一个事件的发生被阻断，那么所有后续事件的发生也会被阻断。这对已提出的用于解释孢子发育中观察到的时间序列的生化模型具有启示意义。
