Dunn G, Torgersen D M, Mandelstam J
J Bacteriol. 1976 Mar;125(3):776-9. doi: 10.1128/jb.125.3.776-779.1976.
The mean volumes of stationary-phase cells of wild-type and asporogenous mutants of Bacillus subtilis have been measured. Mutants blocked at stage 0 of sporulation either produced cells that had the same volume as the developing sporangium or they divided to produce cells of one-half this volume. The order of expression of the genes affected by the mutations in these strains was determined by biochemical characterization and by construction of double sporulation mutants. Mutants that produced small cells were blocked at an earlier stage of sporulation than those that produced large cells. It is suggested that the following dependent sequence must occur before the formation of the prespore spetum: (i) the initiation of sporulation, (ii) a signal to block the final central division site, and (iii) a signal to activate a polar septum site.
已对枯草芽孢杆菌野生型和无芽孢突变体的稳定期细胞平均体积进行了测量。在芽孢形成0期受阻的突变体要么产生与正在发育的芽孢囊体积相同的细胞，要么分裂产生体积为其一半的细胞。通过生化特性分析以及构建双芽孢形成突变体，确定了这些菌株中受突变影响的基因的表达顺序。产生小细胞的突变体在芽孢形成过程中比产生大细胞的突变体受阻于更早阶段。有人提出，在芽孢前隔膜形成之前必须发生以下依赖序列：（i）芽孢形成的起始；（ii）阻断最终中央分裂位点的信号；（iii）激活极性隔膜位点的信号。
