分枝杆菌属中的核酸同源性。
Nucleic acid homology in the genus Mycobacterium.
作者信息
Gross W M, Wayne L G
出版信息
J Bacteriol. 1970 Nov;104(2):630-4. doi: 10.1128/jb.104.2.630-634.1970.
Abstract
Immobilized deoxyribonucleic acid (DNA) preparations from eight species of mycobacteria were reacted with labeled reference DNA from Mycobacterium tuberculosis and M. kansasii. All mycobacteria showed some degree of homology. Immobilized DNA from Pseudomonas multivorans, which has a guanine plus cytosine content within the range established for the mycobacteria, showed no significant binding with either reference system. Relative per cent binding and thermal stability of bound DNA were used as parameters for determining degrees of relatedness among members of the genus. When these relationships were compared with those established by numberical taxonomic methods, a high correlation was found.
摘要
用来自8种分枝杆菌的固定化脱氧核糖核酸（DNA）制剂与来自结核分枝杆菌和堪萨斯分枝杆菌的标记参考DNA进行反应。所有的分枝杆菌都表现出某种程度的同源性。来自多食假单胞菌的固定化DNA，其鸟嘌呤加胞嘧啶的含量在为分枝杆菌确定的范围内，与任何一个参考体系均未显示出明显的结合。结合DNA的相对结合百分比和热稳定性被用作确定该属成员间亲缘关系程度的参数。当将这些关系与通过数值分类学方法确定的关系进行比较时，发现存在高度相关性。
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