Hammersley P A, Taylor D M
Eur J Nucl Med. 1979 Aug 1;4(4):261-70. doi: 10.1007/BF00304881.
The distribution of 67Ga citrate at 24 h post injection has been studied in the normal tissues of the mouse, rat and dog; 13 transplantable mouse tumours and 7 rat tumours have also been examined. The total activities of four lysosomal enzymes, aryl sulphatase, beta-glucuronidase, acid phosphatase and cathepsin-D were measured as well as the incorporation of thymidine-3H and leucine-14C ad indicators of DNA and protein synthesis. The results show a close correlation between 67Ga uptake and lysosomal enzyme activity in the tumours studied, which is an extension of the same relationship for normal tissues. It is suggested that the reported correlation between the uptake of 67Ga and the rate of cellular proliferation is secondary to the primary function of the lysosome in the localisation of the nuclide, lysosomal enzyme activity also being enhanced in situations of increased metabolic activity. A similar relationship appears to occur following administration of 111IN-Bleomycin and 99mTc-Citrate.
已对小鼠、大鼠和犬的正常组织中注射柠檬酸镓67Ga后24小时的分布情况进行了研究；还检查了13种可移植的小鼠肿瘤和7种大鼠肿瘤。测定了四种溶酶体酶（芳基硫酸酯酶、β-葡萄糖醛酸酶、酸性磷酸酶和组织蛋白酶-D）的总活性，以及作为DNA和蛋白质合成指标的胸腺嘧啶核苷-3H和亮氨酸-14C的掺入情况。结果表明，在所研究的肿瘤中，67Ga摄取与溶酶体酶活性之间存在密切相关性，这是正常组织中相同关系的延伸。有人提出，所报道的67Ga摄取与细胞增殖速率之间的相关性是溶酶体在核素定位中的主要功能的次要表现，在代谢活性增加的情况下，溶酶体酶活性也会增强。注射铟111博来霉素和枸橼酸锝99mTc后似乎也出现类似关系。
