Sherman L A, Haselkorn R
J Virol. 1970 Dec;6(6):834-40. doi: 10.1128/JVI.6.6.834-840.1970.
Host and viral deoxyribonucleic acid (DNA) metabolism in LPP-1-infected Plectonema boryanum was studied by equilibrium centrifugation in CsCl gradients. Approximately 50% of the host DNA is degraded to acid-soluble material between 3 and 7 hr after infection. Most of the acid-soluble product is reincorporated into viral DNA. Incorporation of exogenous (3)H-adenine into viral DNA can be detected very early after infection (within the first 2 hr), but the bulk of viral DNA synthesis occurs between 6 and 8 hr. Both the breakdown of host DNA and the synthesis of viral DNA require protein synthesis during the first few hours of infection.
通过在氯化铯梯度中进行平衡离心,研究了感染LPP - 1的鞘丝藻中宿主和病毒脱氧核糖核酸(DNA)的代谢情况。感染后3至7小时内,约50%的宿主DNA被降解为酸溶性物质。大部分酸溶性产物重新掺入病毒DNA中。感染后很早就能检测到外源(3)H - 腺嘌呤掺入病毒DNA(在最初2小时内),但大部分病毒DNA合成发生在6至8小时之间。在感染的最初几个小时内,宿主DNA的分解和病毒DNA的合成均需要蛋白质合成。