噬菌体DNA合成所必需的一种T7核酸内切酶的结构基因。
The structural gene for a T7 endonuclease essential for phage DNA synthesis.
作者信息
Center M S, Studier F W, Richardson C C
出版信息
Proc Natl Acad Sci U S A. 1970 Jan;65(1):242-8. doi: 10.1073/pnas.65.1.242.
Abstract
Infection of Escherichia coli with bacteriophage T7 results in the appearance of an endonuclease activity capable of hydrolyzing both double-and single-stranded DNA. Treatment with chloramphenicol prevents the induction of the endonuclease. Amber mutants of phage T7 defective in gene 3 are unable to produce the enzyme after infection of the nonpermissive host, and mutants that produce a heat-labile endonuclease were found, indicating that this gene is the structural gene for the enzyme. Gene 3 mutants synthesize only a limited amount of DNA. In addition, they are defective in carrying out the degradation of host DNA, suggesting that the gene 3 endonuclease is involved in this function.
摘要
用噬菌体T7感染大肠杆菌会导致一种核酸内切酶活性的出现，该活性能够水解双链和单链DNA。用氯霉素处理可阻止核酸内切酶的诱导。噬菌体T7基因3有缺陷的琥珀突变体在感染非允许宿主后无法产生该酶，并且发现了产生热不稳定核酸内切酶的突变体，这表明该基因是该酶的结构基因。基因3突变体仅合成有限量的DNA。此外，它们在进行宿主DNA降解方面存在缺陷，这表明基因3核酸内切酶参与了这一功能。
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