Herlyn D, Herlyn M, Steplewski Z, Koprowski H
Eur J Immunol. 1979 Aug;9(8):657-9. doi: 10.1002/eji.1830090817.
Hybridoma-derived monoclonal anti-melanoma antibodies and anti-colorectal carcinoma antibodies were found to mediate in vitro antibody dependent cell-mediated cytotoxicity (ADCC) reactions against melanoma and colorectal carcinoma cells, respectively. The antigen(s) detected in ADCC on melanoma cells maintained for more than one hundred passages in tissue culture were also found on two recently established melanoma cell lines. These antigens were not detected on skin fibroblasts of the same patients from whom the melanomas were obtained. The ADCC reactivities of anti-melanoma and anti-colorectal carcinoma antibodies were found to be specific for melanoma cells and colorectal carcinoma cells, respectively.
发现杂交瘤衍生的抗黑色素瘤单克隆抗体和抗结肠直肠癌抗体分别介导针对黑色素瘤细胞和结肠直肠癌细胞的体外抗体依赖性细胞介导的细胞毒性（ADCC）反应。在组织培养中传代超过一百代的黑色素瘤细胞上通过ADCC检测到的抗原，在两个最近建立的黑色素瘤细胞系上也被发现。在获取黑色素瘤的同一患者的皮肤成纤维细胞上未检测到这些抗原。发现抗黑色素瘤和抗结肠直肠癌抗体的ADCC反应分别对黑色素瘤细胞和结肠直肠癌细胞具有特异性。
