Buckett W R
Eur J Pharmacol. 1979 Sep 15;58(2):169-78. doi: 10.1016/0014-2999(79)90009-8.
Peripheral stimulation was applied to mice by mild caudal electrostimulation, by mechanical pressure or by footshock for 30 sec, before testing on a 52 degrees C hot plate. Reaction times to paw lick and to escape from the hot plate were recorded. Analgesia could be elicited and measured by these procedures. It was of short duration, declining in a minute, and was antagonized by low doses of naloxone. The analgesia measured by the escape reaction time could be elicited after multiple caudal electrostimulation as well as in morphine-tolerant mice, and it could still be reversed by naloxone. An opioid link is thus involved in this phenomenon, which also supports the notion of more than one opioid pathway existing in the brain. The short period of analgesic cover afforded in the face of noxious stimuli would permit aversive action to be taken in nature and thus might represent the prime functional role of enkephalins in the brain.
在对小鼠进行52摄氏度热板测试之前，通过轻度尾部电刺激、机械压力或足部电击对其施加外周刺激30秒。记录舔爪和逃离热板的反应时间。通过这些程序可以诱发并测量镇痛效果。其持续时间较短，一分钟内减弱，并且会被低剂量的纳洛酮拮抗。通过逃避反应时间测量的镇痛效果，在多次尾部电刺激后以及吗啡耐受的小鼠中均可诱发，并且仍可被纳洛酮逆转。因此，这一现象涉及阿片类物质联系，这也支持了大脑中存在不止一条阿片类途径的观点。面对有害刺激时提供的短暂镇痛覆盖期，将使机体在自然环境中采取厌恶行动，因此可能代表脑啡肽在大脑中的主要功能作用。
