淋巴细胞性脉络丛脑膜炎病毒及病毒核糖核酸的密度梯度离心研究
Density gradient centrifugation studies on lymphocytic choriomeningitis virus and on viral ribonucleic acid.
作者信息
Pedersen I R
出版信息
J Virol. 1970 Oct;6(4):414-20. doi: 10.1128/JVI.6.4.414-420.1970.
Abstract
Lymphocytic choriomeningitis (LCM) virus, Traub strain, was purified from BHK-21 tissue culture medium. The virus was then analyzed by equilibrium centrifugation and rate zonal centrifugation in sucrose gradients. A buoyant density in sucrose of 1.18 g/ml was found and the S(20, w) value was estimated to be about 470 to 500S. Furthermore, the (3)H-uridine-labeled ribonucleic acid (RNA) from virus was extracted from LCM virus and analyzed by rate zonal centrifugation. Two major and one minor single-stranded RNA components were found with sedimentation coefficients of 28, 22, and 18S.
摘要
从BHK - 21组织培养基中纯化出淋巴细胞性脉络丛脑膜炎（LCM）病毒，特劳伯毒株。然后通过蔗糖梯度平衡离心和速率区带离心对该病毒进行分析。发现其在蔗糖中的浮力密度为1.18 g/ml，S(20, w)值估计约为470至500S。此外，从LCM病毒中提取经³H - 尿苷标记的核糖核酸（RNA），并通过速率区带离心进行分析。发现了两个主要的和一个次要的单链RNA组分，沉降系数分别为28、22和18S。
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