Pedersen I R, Konigshofer E P
J Virol. 1976 Oct;20(1):14-21. doi: 10.1128/JVI.20.1.14-21.1976.
Disruption of purified lymphocytic choriomeningitis (LCM) virus with Nonidet P-40 in 0.5 M KCl followed by sucrose gradient centrifugation in 0.3 M KCl led to the isolation of two viral nucleoproteins (RNPs) as well as 40S and 60S ribosomal subunits. The largest viral RNP sedimented heterogenously at 123S to 148S and was associated with 23S and 31S viral RNA. The other viral RNP sedimented at 83S and was associated with 23S viral RNA. The buoyant density in CsCl was determined to be 1.32 g/cm3 for the viral RNP. Densities of 1.52 and 1.60 g/cm3 were determined for the 40S and 60S subunits, similar to those of the BHK-21 cells subunits dissociated by 0.5 M KCl. The viral RNPs were partly sensitive to RNase.
用去氧胆酸钠（Nonidet P - 40）在0.5M氯化钾中裂解纯化的淋巴细胞性脉络丛脑膜炎（LCM）病毒，随后在0.3M氯化钾中进行蔗糖梯度离心，分离出两种病毒核蛋白（核糖核蛋白，RNPs）以及40S和60S核糖体亚基。最大的病毒核糖核蛋白在123S至148S处呈异质性沉降，并与23S和31S病毒RNA相关联。另一种病毒核糖核蛋白在83S处沉降，并与23S病毒RNA相关联。病毒核糖核蛋白在氯化铯中的浮力密度测定为1.32 g/cm³。40S和60S亚基的密度分别测定为1.52和1.60 g/cm³，与用0.5M氯化钾解离的BHK - 21细胞亚基的密度相似。病毒核糖核蛋白对核糖核酸酶部分敏感。
