从淋巴细胞性脉络丛脑膜炎病毒中分离出的核糖核蛋白和核糖体的特性分析
Characterization of ribonucleoproteins and ribosomes isolated from lymphocytic choriomeningitis virus.
作者信息
Pedersen I R, Konigshofer E P
出版信息
J Virol. 1976 Oct;20(1):14-21. doi: 10.1128/JVI.20.1.14-21.1976.
Abstract
Disruption of purified lymphocytic choriomeningitis (LCM) virus with Nonidet P-40 in 0.5 M KCl followed by sucrose gradient centrifugation in 0.3 M KCl led to the isolation of two viral nucleoproteins (RNPs) as well as 40S and 60S ribosomal subunits. The largest viral RNP sedimented heterogenously at 123S to 148S and was associated with 23S and 31S viral RNA. The other viral RNP sedimented at 83S and was associated with 23S viral RNA. The buoyant density in CsCl was determined to be 1.32 g/cm3 for the viral RNP. Densities of 1.52 and 1.60 g/cm3 were determined for the 40S and 60S subunits, similar to those of the BHK-21 cells subunits dissociated by 0.5 M KCl. The viral RNPs were partly sensitive to RNase.
摘要
用去氧胆酸钠（Nonidet P - 40）在0.5M氯化钾中裂解纯化的淋巴细胞性脉络丛脑膜炎（LCM）病毒，随后在0.3M氯化钾中进行蔗糖梯度离心，分离出两种病毒核蛋白（核糖核蛋白，RNPs）以及40S和60S核糖体亚基。最大的病毒核糖核蛋白在123S至148S处呈异质性沉降，并与23S和31S病毒RNA相关联。另一种病毒核糖核蛋白在83S处沉降，并与23S病毒RNA相关联。病毒核糖核蛋白在氯化铯中的浮力密度测定为1.32 g/cm³。40S和60S亚基的密度分别测定为1.52和1.60 g/cm³，与用0.5M氯化钾解离的BHK - 21细胞亚基的密度相似。病毒核糖核蛋白对核糖核酸酶部分敏感。
相似文献
1
Characterization of ribonucleoproteins and ribosomes isolated from lymphocytic choriomeningitis virus.从淋巴细胞性脉络丛脑膜炎病毒中分离出的核糖核蛋白和核糖体的特性分析
J Virol. 1976 Oct;20(1):14-21. doi: 10.1128/JVI.20.1.14-21.1976.
2
Properties of virions and ribonucleoproteins of Venezuelan equine encephalomyelitis virus.委内瑞拉马脑炎病毒的病毒粒子和核糖核蛋白特性
Acta Virol. 1977 May;21(3):213-221.
3
Semliki Forest virus capsid protein associates with the 60S ribosomal subunit in infected cells.塞姆利基森林病毒衣壳蛋白与受感染细胞中的60S核糖体亚基相关联。
J Virol. 1976 Oct;20(1):203-10. doi: 10.1128/JVI.20.1.203-210.1976.
4
Ribonucleoprotein of avian infectious bronchitis virus.禽传染性支气管炎病毒核糖核蛋白
J Gen Virol. 1981 Mar;53(Pt 1):67-74. doi: 10.1099/0022-1317-53-1-67.
5
Different classes of ribonucleic acid isolated from lymphocytic choriomeningitis virus.从淋巴细胞性脉络丛脑膜炎病毒中分离出的不同种类核糖核酸。
J Virol. 1973 Mar;11(3):416-23. doi: 10.1128/JVI.11.3.416-423.1973.
6
Density gradient centrifugation studies on lymphocytic choriomeningitis virus and on viral ribonucleic acid.淋巴细胞性脉络丛脑膜炎病毒及病毒核糖核酸的密度梯度离心研究
J Virol. 1970 Oct;6(4):414-20. doi: 10.1128/JVI.6.4.414-420.1970.
7
Viral proteins and RNAs in BHK cells persistently infected by lymphocytic choriomeningitis virus.被淋巴细胞性脉络丛脑膜炎病毒持续感染的BHK细胞中的病毒蛋白和RNA。
J Virol. 1983 Oct;48(1):262-70. doi: 10.1128/JVI.48.1.262-270.1983.
8
Isolation of ribosome-like sturctures from Pichinde virus.从皮钦德病毒中分离核糖体样结构。
J Gen Virol. 1975 Jan;26(1):21-31. doi: 10.1099/0022-1317-26-1-21.
9
Mitochondrial and cytoplasmic ribosomes from mammalian tissues. Further characterization of ribosomal subunits and validity of buoyant-density methods for determination of the chemical composition and partial specific volume of ribonucleoprotein particles.哺乳动物组织中的线粒体核糖体和细胞质核糖体。核糖体亚基的进一步表征以及用于测定核糖核蛋白颗粒化学成分和偏比容的浮力密度法的有效性。
Biochem J. 1977 Nov 15;168(2):245-59. doi: 10.1042/bj1680245.
10
Biochemical composition of lymphocytic choriomeningitis virus interfering particles.淋巴细胞性脉络丛脑膜炎病毒干扰颗粒的生化组成。
J Gen Virol. 1981 Aug;55(Pt 2):475-9. doi: 10.1099/0022-1317-55-2-475.
引用本文的文献
1
References.参考文献。
Perspect Med Virol. 1986;2:209-245. doi: 10.1016/S0168-7069(08)70043-0. Epub 2008 May 29.
2
Encapsidation of host-derived factors correlates with enhanced infectivity of Sindbis virus.宿主来源因子的囊膜化与 Sindbis 病毒感染力的增强相关。
J Virol. 2013 Nov;87(22):12216-26. doi: 10.1128/JVI.02437-13. Epub 2013 Sep 4.
3
Proteomics analysis unravels the functional repertoire of coronavirus nonstructural protein 3.蛋白质组学分析揭示了冠状病毒非结构蛋白3的功能库。
J Virol. 2008 Jun;82(11):5279-94. doi: 10.1128/JVI.02631-07. Epub 2008 Mar 26.
4
Ambisense RNA genomes of arenaviruses and phleboviruses.沙粒病毒和白蛉病毒的双义RNA基因组。
Adv Virus Res. 1986;31:1-51. doi: 10.1016/s0065-3527(08)60261-4.
5
Recombination between temperature-sensitive mutants of the arenavirus Pichinde.沙粒病毒皮钦德温度敏感突变体之间的重组。
J Virol. 1977 Nov;24(2):712-5. doi: 10.1128/JVI.24.2.712-715.1977.
本文引用的文献
1
Some properties of influenza virus nucleocapsids.流感病毒核衣壳的一些特性。
J Virol. 1969 Sep;4(3):219-25. doi: 10.1128/JVI.4.3.219-225.1969.
2
Buoyant densities of cytoplasmic ribonucleoprotein particles of mammalian cells: distinctive character of ribosome subunits and the rapidly labeled components.哺乳动物细胞胞质核糖核蛋白颗粒的浮力密度：核糖体亚基和快速标记成分的独特特征。
J Mol Biol. 1966 Apr;16(2):255-68. doi: 10.1016/s0022-2836(66)80171-7.
3
Isolation and properties of Newcastle disease virus nucleocapsid.新城疫病毒核衣壳的分离与特性
J Virol. 1968 Mar;2(3):248-55. doi: 10.1128/JVI.2.3.248-255.1968.
4
Preparation of mammalian polyribosomes with the detergent Nonidet P-40.用去污剂Nonidet P-40制备哺乳动物多核糖体
Biochim Biophys Acta. 1967 Nov 21;149(1):302-4. doi: 10.1016/0005-2787(67)90715-0.
5
Optimal conditions for counting of precipitated 3H-RNA on glass-fiber filters.玻璃纤维滤膜上沉淀的3H-RNA计数的最佳条件。
Anal Biochem. 1970 Sep;37(1):178-82. doi: 10.1016/0003-2697(70)90275-7.
6
Structure and function of mammalian ribosomes. I. Isolation and characterization of active liver ribosomal subunits.哺乳动物核糖体的结构与功能。I. 活性肝脏核糖体亚基的分离与特性分析。
J Mol Biol. 1970 Oct 14;53(1):1-19. doi: 10.1016/0022-2836(70)90042-2.
7
Conjugation of fluorescein isothiocyanate to antibodies. I. Experiments on the conditions of conjugation.异硫氰酸荧光素与抗体的结合。I. 结合条件的实验。
Immunology. 1970 Jun;18(6):865-73.
8
Dissociation of mammalian polyribosomes into subunits by puromycin.嘌呤霉素使哺乳动物多核糖体解离为亚基。
Proc Natl Acad Sci U S A. 1971 Feb;68(2):390-4. doi: 10.1073/pnas.68.2.390.
9
Formation of active hybrids from subunits of muscle ribosomes from normal and diabetic rats.正常和糖尿病大鼠肌肉核糖体亚基形成活性杂种。
Proc Natl Acad Sci U S A. 1968 Jun;60(2):569-74. doi: 10.1073/pnas.60.2.569.
10
Lymphocytic choriomeningitis virus RNAs.淋巴细胞性脉络丛脑膜炎病毒核糖核酸
Nat New Biol. 1971 Nov 24;234(47):112-4. doi: 10.1038/newbio234112a0.
Zotero 插件