Biberfeld G
Clin Exp Immunol. 1971 Feb;8(2):319-33.
Complement-fixing (CF) antibodies against brain tissue antigens were demonstrated in seven cases of infection with neurological complications. However, forty-three out of fifty-four cases (80%) without neurological symptoms also acquired antibodies to brain during the course of infection. 75% of the patients with antibodies to brain also had antibodies against human lung and liver, but the antibody titres to the latter tissue antigens were usually lower than against brain. The reactivity of antisera to brain and other tissues was significantly reduced by prior absorption with antigen, indicating the presence of a related antigen in organisms and these tissues. Fractionation by density gradient centrifugation of thirty-three sera from twenty-six cases showed the antibodies to brain to be of the IgM class exclusively in twenty-four cases while the CF antibodies to were associated with both IgM and IgG. The brain antigen reacting in the CF test with antisera could be extracted with chloroform–methanol in the same way as the crude lipid CF antigen of .
在7例伴有神经并发症的感染病例中，检测到了针对脑组织抗原的补体结合（CF）抗体。然而，54例无神经症状的病例中，有43例（80%）在感染过程中也产生了针对脑的抗体。75%的脑抗体阳性患者同时也有针对人肺和肝的抗体，但针对后一种组织抗原的抗体滴度通常低于针对脑的抗体滴度。用抗原预先吸收后，抗血清对脑和其他组织的反应性显著降低，表明在生物体和这些组织中存在相关抗原。对26例患者的33份血清进行密度梯度离心分离，结果显示，24例患者中针对脑的抗体仅为IgM类，而针对[此处原文缺失相关内容]的CF抗体则与IgM和IgG均有关。CF试验中与抗血清发生反应的脑抗原，可用氯仿 - 甲醇以与[此处原文缺失相关内容]的粗脂质CF抗原相同的方式提取。
