von Fellenberg R
Immunology. 1971 Jun;20(6):963-72.
Rabbit antisera to mouse spleen cells and to thymocytes have been analysed by microcomplement fixation and double gel diffusion. The antisera fixed complement more efficiently with the native particulate fraction than with the soluble fraction of mouse spleen cells. Structural antigens could be solubilized partly with hypertonic salt solutions. The solubilization of structural antigens could be shown by a decrease of the antigenic activity of the particulate fraction after the extraction and a concomitant appearance of the antigenic activity in the solubilized fractions. The solubilized fractions were able to induce antibodies to structural antigens, but antibodies to cell sap antigens were also induced to a lesser extent. No gel precipitation could be observed between the tested antisera and the solubilized fractions.
已通过微量补体结合试验和双向凝胶扩散法分析了兔抗小鼠脾细胞和胸腺细胞血清。与小鼠脾细胞的可溶性部分相比,这些抗血清与天然颗粒部分结合补体的效率更高。结构抗原可部分用高渗盐溶液溶解。提取后颗粒部分抗原活性降低,同时溶解部分出现抗原活性,这可证明结构抗原的溶解。溶解部分能够诱导产生针对结构抗原的抗体,但也能在较小程度上诱导产生针对细胞液抗原的抗体。在所测试的抗血清与溶解部分之间未观察到凝胶沉淀。