Lance E M, Ford P J, Ruszkiewicz M
Immunology. 1968 Oct;15(4):571-80.
Subcellular preparations of mouse thymocytes were compared with respect to their power to raise in rabbits antisera which prolonged the survival of skin homografts. Of the fractions studied thymocyte membrane' gave the most consistently good results, whereas thymocyte mitochondria' gave rise to sera which were toxic in high dose. Whole mouse liver homogenate or liver subcellular fractions did not elicit potent antisera. It is concluded that thymocyte `membrane' best fulfils the requirements for an ideal material for immunization thus far studied and provides a convenient starting point for further purification.
对小鼠胸腺细胞的亚细胞制剂进行了比较，以研究它们在兔体内产生能延长皮肤同种异体移植存活时间的抗血清的能力。在所研究的组分中，胸腺细胞“膜”产生的结果最为一致且良好，而胸腺细胞“线粒体”产生的血清在高剂量时具有毒性。全小鼠肝匀浆或肝亚细胞组分未能引发高效价抗血清。结论是，胸腺细胞“膜”最符合迄今为止所研究的理想免疫材料的要求，并为进一步纯化提供了便利的起始点。
